TYPE 1 COPPER COORDINATION IN THE ABSENCE OF SULFUR

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们表达和纯化了铜绿假单胞菌天青素突变体，其还原电位和EPR谱类似于1型(T1)或蓝铜？尽管缺乏硫酸盐连接，但位点。到目前为止，研究表明硫代化合物对T1配位是必要的。与正在进行的电子吸收、EPR和电子转移动力学研究相结合，我们希望使用XAS和EXAFS分析来阐明我们特殊的EPR谱的来源，并评估氧化/还原过程中的位置重组。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have expressed and purified mutants of Pseudomonas aeruginosa azurins which possess reduction potentials and EPR spectra akin to type 1 (T1) or ?blue copper? sites despite lack of thiolate ligation. To date research has indicated the necessity of thiolates for T1 coordination. In concert with ongoing electronic absorption, EPR, and electron transfer kinetics studies, we wish to use XAS and EXAFS analysis to elucidate the origin of our peculiar EPR spectra as well as to assess site reorganization during oxidation/reduction processes.