PROTEINS IN MALARIAL AND TUBERCULOSIS INFECTIONS AND IN BACTERIAL ACQUIRED IMMUN

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 博世实验室对疟疾蛋白感兴趣，这些蛋白与寄生虫侵入或离开人类肝脏、红细胞以及蚊子媒介中的各种细胞类型有关。我们的目标是通过基于结构的药物设计和X射线结晶学相结合的方法，通过关键蛋白质的小分子抑制剂来削弱寄生虫的增殖。普里格实验室对疟疾和结核病关键蛋白的结构生物学感兴趣。目前的蛋白质晶体包括与病原体在结核病中的持久性和在疟疾中的性别分化有关的蛋白质。贝利实验室对原核生物中一种新鉴定的获得性免疫系统进行结构功能研究，这与真核生物RNAi的一些基本原理相同。通过对晶体结构和功能数据的分析，我们希望能弄清这种免疫的分子机制。为此，我们获得了所涉及的几种蛋白质的晶体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Bosch lab is interested in malarial proteins involved in invasion and egress of the parasite into or from various cell types in the human liver, red blood cells as well as in the Mosquito vector. We aim at impairing the parasite's proliferation through small molecule inhibitors for key proteins through structure based drug design in conjunction with x-ray crystallography. The Prigge lab is interested in the structural biology of key proteins from malaria and tuberculosis. Current protein crystals include proteins involved in pathogen persistence in tuberculosis and sexual differentiation in malaria. The Bailey lab performs structure function studies of a newly characterized acquired immune system in prokaryotes, which shares some of the basic principles of eukaryotic RNAi. Through the analysis of crystal structures and functional data we hope to discern the molecular mechanisms underpinning this immunity. To this end we have obtained crystals of several of the proteins involved.