IMPROVED QUANTIFICATION, COVERAGE/DYNAMIC RANGE, SENSITIVITY, AND THROUGHPUT

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 核心2寻求通过大量的技术进步来改进蛋白质组分析。这些进步是通过将能力扩展到包括非常规纳克尺寸和更小的样品，通过提高MS测量的动态范围，通过应用分析完整蛋白质的新方法，通过高灵敏度的磷酸蛋白质组图谱，通过样品处理的进步，通过改进氧-18的稳定同位素标记并将其延伸到LC-SRM测量，通过高分辨率FAIMS的开发，通过应用iTRAQ定量，通过开发磷酸肽的多重定量策略，通过提高生物流体蛋白质组的覆盖率，通过进一步提高MS的灵敏度，以及通过高通量2D Nano-LC-MS，已经实现了这些进步。提供了促进向群体蛋白质组学范式转变的NCRR补充剂的描述。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core 2 seeks to improve proteome analyses through a multitude of technological advancements. These advancements have been achieved by extending the capabilities to include non-conventional nanogram-sized and smaller samples, by enhancing the dynamic range of the MS measurements, by applying new methods of analyzing intact proteins, by high sensitivity phosphoproteome profiling, by advancements in sample processing, by improving stable isotope labeling with oxygen-18 and its extension to LC-SRM measurements, by high resolution FAIMS development, through the application of iTRAQ quantification, by developing a multiplexed quantification strategy for phosphopeptides, by improving biofluid proteome coverage, by making additional advances in MS sensitivity, and by high throughput 2D nano-LC-MS. A description of NCRR supplements that facilitate the paradigm shift to population proteomics is provided.