ULTRASTRUCTURAL ANALYSIS OF MEMBRANE-ASSOCIATED COMPLEXES IN S CEREVISIAE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 Septins是由类似基因编码的GTP结合蛋白，几乎在所有被研究的真核生物的基因组中都能找到。这些蛋白质参与质膜内隔室的重塑和划分，例如在 细胞质分裂(Finger，2005)和神经元中树突棘的成熟(Caudron和Barral，2009)。单细胞真核生物酿酒酵母(面包酵母)编码7个Septin基因，智人编码14个(潘等人，2007)。任何给定的真核细胞类型都表达多组间隔蛋白，通常以细胞类型和发育阶段特有的方式表达。在所有已知的情况下，这些基因的蛋白质产物以确定的顺序关联并组装成线性阵列(McMurray和Thorner，2008a；Weirich等人，2008)。此外，得到的线型杂低聚物(“棒”)是一种能够进行端到端聚合从而形成细丝的构建块。众所周知，这些细丝与细胞分裂有关，但仅靠荧光显微镜还不能揭示足够的细节来彻底了解确切的机制。我们建议使用相关的荧光和X射线成像来确定酿酒酵母中每个隔质的位置。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Septins are GTP-binding proteins encoded by paralogous genes found in the genomes of nearly every eukaryote examined. These proteins are involved in the remodeling of and demarcation of compartments within the plasma membrane, for example during cytokinesis (Finger, 2005) and in the maturation of dendritic spines in neurons (Caudron and Barral, 2009). Unicellular eukaryote Saccharomyces cerevisiae (baker's yeast) encodes seven septin genes, and Homo sapiens fourteen (Pan et al., 2007). Any given eukaryotic cell type expresses multiple sets of septins, often in a cell type- and developmental stage-specific manner. In all cases known, the protein products of those genes associate in a defined order and assemble into a linear array (McMurray and Thorner, 2008a; Weirich et al., 2008). Moreover, the resulting linear hetero-oligomer ("rod") is a building block that has the capacity to undergo end-on-end polymerization, thereby forming filaments. It is known that these filaments are involved with cell division, but fluorescence microscopy alone has not revealed sufficient details to thoroughly understand the exact mechanism. We propose to use correlated fluorescence and x-ray imaging to determine the location of each of the septins in the yeast, Saccharomyces cerevisiae.