A cryo-electron microscopy training and sample-preparation research proposal

描述（由申请人提供）：PI正在开发一种“分子显微镜”，能够拍摄单个大分子的纳米分辨率磁共振图像。为了支持这项工作，PI将接受最先进的低温电子显微镜的培训，并将开展研究，以开发在低温真空中制备脆弱生物大分子的改进方法。在样品制备方面提出的研究将使低温电子显微镜和纳米分辨率磁共振成像更容易研究更大范围的样品。

DESCRIPTION (provided by applicant): The PI is developing a "molecular microscope" capable of taking nanometer-resolution magnetic resonance images of individual macromolecules. In support of this work, the PI will be trained in state-of-the- art cryo-electron microscopy, and will carry out research to develop improved approaches for preparing fragile biomacromolecules for study at low temperatures in vacuum. The proposed research in sample preparation will make it much easier to study a wider range of samples by both cryo-electron microscopy and nanometer-resolution magnetic resonance imaging.