BIOBANK CORE

生物库核心将协助项目成员实验室积累和集中胰腺癌研究至关重要的生物资源。我们将利用我们直接获得人类手术标本的优势，为P01研究人员提供在组织学(与实验病理学核心合作)和基因水平上经过广泛表征的生物材料。核心将收集、启动、维护、存档和记录所有人类PDAC生物资源。这些材料包括从原发和转移性人胰腺癌标本中建立的连续传代的异种移植物，以及PDAC细胞系的资料库。异种移植瘤和细胞系已经进行了高分辨率的aCGH和表达谱分析，使研究人员能够检索到具有适当基因组谱的所需生物材料。总而言之，这些资源应该促进分子研究，并为进行实验研究提供标准化的细胞系统。生物库核心成员在实施用于测试的异种移植系统方面拥有丰富的经验 新的治疗方法以及分子和生物学研究。该核心将与P01的项目密切互动，以提供生物材料，并协助设计和解释使用异种移植物和原代细胞的实验。核心将努力确保高水平的标本质量控制和协议标准化，以确保P01项目之间的成果有效转化。

The BioBank Core will assist project member laboratories in the accrual and centralization of biologic resources that are critically required for pancreatic cancer research. We will take advantage of our immediate access to human surgical specimens to provide P01 investigators with biological materials that have been subjected to extensive characterization at the histologic (in collaboration with the Experimental Pathology Core) and genetic levels. The Core will collect, initiate, maintain, archive and record all human PDAC biologic resources. These materials include serially passaged xenografts created from primary and metastatic human pancreatic cancer specimens and a repository of PDAC cell lines. The xenograft tumors and cell lines have been subjected to high-resolution aCGH and expression profiling, enabling investigators to retrieve needed biologic materials with an appropriate genomic profile. Together, these resources should facilitate molecular studies and provide standardized cellular systems for performing experimental studies. Members of the BioBank Core have extensive experience in implementation of xenograft systems for testing novel therapeutic approaches and for molecular and biological studies. The Core will interact closely with the projects of the P01 to provide the biological materials and aid in the design and interpretation of experiments employing the xenografts and primary cells. The Core will seek to ensure a high level of quality control of specimens and standardization of protocols to ensure the effective translation of results between P01 projects.