Production, Validation and Distribution of the Xenopus ORFeome

描述（由申请人提供）：我们建议生成，验证和分发Xenopus ORFeome，我们将其定义为一套完全测序，验证的Xenopus cDNA克隆，其中包含基因组中编码的每个开放阅读框（ORF），其格式是任何ORF序列都可以通过重组轻松转移到不同的表达载体阵列中。这一套试剂将大大减少在整个爪蟾群落进行的无数功能分析中表征任何蛋白质的时间。但最重要的是，ORFeome将允许高通量的体内功能基因组筛选，这是目前不可行的方式，并将利用非洲爪蟾系统的特殊优势。这些克隆将不受限制地提供给全世界的研究人员。爪蟾社区完全支持这个项目，在最近的一次PI会议上（Lake Louise， 2010年9月），ORFeome被选为最优先需要的爪蟾资源。

DESCRIPTION (provided by applicant): We propose to generate, validate and distribute a Xenopus ORFeome, which we define as a fully sequenced, validated set of Xenopus cDNA clones containing one each of every open reading frame (ORF) encoded in the genome, in a format in which any ORF sequence can be easily transferred using recombineering into a diverse array of expression vectors. This one set of reagents will greatly decrease the time to characterize any protein in the myriad of functional assays carried out by the entire Xenopus community. But most importantly, an ORFeome set will allow high-throughput in vivo functional-genomic screening in manner currently not feasible, and will take advantage of a particular strength of the Xenopus system. These clones will be made available, without restriction, to researchers worldwide. The Xenopus community is fully supportive of this project and at a recent PI meeting (Lake Louise, September 2010) the ORFeome was voted as the Top priority of needed Xenopus resources.