Animal Core

甲基苯丙胺滥用研究中心（MARC）动物中心将为该中心服务，创建MA饮用的精选品系，维护该遗传小鼠模型，协助中心所需行为测试的标准化，优先分配和分发小鼠，从商业供应商处购买小鼠，以及维护一个动物跟踪数据库，与MARC生物统计和遗传学中心维护的实验结果数据库一起使用。动物中心将由John Crabbe博士管理，他在开发成瘾相关特征的遗传动物模型方面经验丰富，并将监督MARC所有科学组成部分和新试点项目所需小鼠的维护和供应。动物核心产生的资源也将提供给外部调查人员。克拉布博士将指导有经验的员工；这些员工多年来一直在克拉布博士的实验室里合作工作。所有动物将被安置在AALAC认可的动物设施中，由持牌兽医全程监督。为了避免病毒和细菌污染，有适当的程序，并使用哨兵筛选程序来快速识别污染并避免传播。为了维持大量基因型，谨慎的基因记录保存和鉴定实践已经实施了数年。动物核心的主要优势是与集中开发相关的成本节约；所选系小鼠的行为测试及分布；跨组件8、组件9和组件10的行为测试标准化，而不是扩展到其他用户；受控环境条件；遗传质量控制；从成分pi中去除动物生产负担。有关动物分配的决定将集中并基于一些标准，包括请求日期、请求是否为MARC项目、科学进展以及最后一次分配到该项目的日期。Core将能够在不同的项目中分配产仔，以尽量减少研究中的产仔影响，并增加跨实验关联分析的可能性。核心实践将加强MARC生成的数据。

The Methamphetamine Abuse Research Center (MARC) Animal Core will serve the Center by creating selected lines for MA drinking, maintaining this genetic mouse model, assisting with the standardization of behavioral tests needed by the Center, prioritizing distribution and distributing mice, purchasing mice from commercial suppliers, and maintaining an animal tracking database to be used in conjunction with experimental results databases maintained by the MARC Biostatistics and Genetics Core. The Animal Core will be managed by Dr. John Crabbe, who is experienced in developing genetic animal models of addiction-related traits and will oversee maintenance and provision of mice for all Scientific Components of the MARC and for new pilots on an as needed basis. Animal Core generated resources will also be made available to outside investigators. Dr. Crabbe will supervise an experienced staff; members of that staff have been working collaboratively or within Dr. Crabbe's lab for many years. All animals will be housed in AALAC accredited animal facilities under the full-time supervision of a licensed veterinarian. Procedures are in place for the avoidance of viral and bacterial contamination, and a sentinel screening program is used to quickly identify contamination and avoid spread. Careful genetic record-keeping and identification practices for the maintenance of a large number of genotypes have been in place for several years. Key advantages of the Animal Core are cost savings associated with centralized development; behavioral testing and distribution of the selected line mice; standardization of behavioral testing across Components 8 and 9 and 10 than can be extended to other users; controlled environmental conditions; genetic quality control; removal of animal production burden from Component PIs. Decisions about animal distribution will be centralized and based on a number of criteria, including date of request, whether the request is for a MARC project, scientific progress, and date of last distribution to that project. The Core will be able to distribute littermates across projects to minimize litter effects within studies, and increase the potential for cross-experiment association analyses. Core practices will strengthen the data generated by the MARC.