Data Management and Resource Dissemination Core

该核心的目标是提供一个中央系统，用于管理关于结核分枝杆菌（Mtb）基因功能的信息并向公众展示。整个项目预计将产生大量与鉴定结核分枝杆菌基因组中基因功能相关的数据。特别是，由每个组成项目生成的主要基因组规模数据类型包括：敲除突变体中转座子插入文库的测序数据；用于基因相互作用定位、表型和抑制突变体的全基因组测序以及细菌突变体的基因表达分析（RNA-Seq）的其他基因或基因组区域的重要性变化的统计估计。将建立一个网站，提供对所产生的原始数据的访问，以及派生信息的可视化显示（热图、基因组浏览器、转座子插入密度图等）。此外，我们将专注于提供数据的统计分析，例如严格识别基因表达和基因必要性的统计显著变化。所有这些数据将被结合起来进行基因功能的推断，对此我们将使用基因本体（gene Ontology， GO）术语。该网站将提供支持每个推论的所有证据来源的链接，以及按GO术语分组/计数/搜索基因的工具。

The goal of this Core is to provide a central system for managing information generated about functions of genes in M. tuberculosis (Mtb) and presenting it to the public. The overall Program Project is anticipated to generate a large amount of data related to identification of functions for genes in the Mtb genome. In particular, major types of genome-scale data to be generated by each of the component projects include: data from sequencing transposon-insertion libraries in knock-down mutants; statistical estimates of changes in essentiality of other genes or regions of the genome for genetic interaction mapping, whole-genome sequencing of phenotypic and suppressor mutants, and gene expression analysis (RNA-Seq) in bacterial mutants. A web site will be developed to provide access to the raw data generated, as well as visual display of derived information (heat maps, genome browser, plots of transposon insertion density, etc.). In addition, we will focus on providing statistical analysis of the data, such as rigorous identification of statistically significant changes in gene expression and gene essentiality. All this data will be combined to make inferences of gene function, for which we will use Gene Ontology (GO) terms. The website will provides links to all sources of evidence supporting each inference, as well as tools for grouping/counting/searching genes by GO term.