Cell and Immunohistochemistry Core

在过去的6年中，我们继续协调绵羊血管和人脐带的分布，并分离，纯化和表征UAEC和HUVEC-CS细胞，用于所有四个项目。所有这些细胞现在都有很好的特征。最近，我们还建立了原代HUVE和HUAEC，所有原代人内皮细胞都保留了许多正常的体内生理功能，例如对生长因子的反应和负责血管舒张剂产生（NO）的关键基因的表达，这是我们将在本计划项目资助中确定的细胞反应。C区已经冻结了很多瓶UAEC HUVEC和HUAEC储备液用于当前应用。我们还建立了标准的免疫细胞化学方案，用于比色和荧光显微镜分析，以及用于间隙连接超微结构形态的透射电子显微镜（TEM）分析。因此，通过作为核心，我们将提供基本的细胞供应，在NO检测和免疫细胞化学，TEM和质量保证方面急需的技术专长，从而有助于直接比较来自不同项目和细胞类型之间的数据。

During the last 6 years we have continued to coordinate distribution of ovine vessels and human umbilical cords, and isolate, purify, and characterize UAEC and HUVEC-CS cells for usage of all four projects. All of these cells are now very well characterized. Recently, we have also established primary HUVE and HUAEC, All primary human endothelial cells retain many normal in vivo physiologic functions such as in responses to growth factors and expression of key genes responsible for vasodilator production (NO), which are the cell responses we will determine in this Program Project Grant. Core C has frozen down many vials of UAEC, HUVEC, and HUAEC stocks for usage of the current application. We have also established standard immunocytochemistry protocols for colorimetric and fluorescent microscopy analysis, and transmission electron microscopy (TEM) analysis for Gap junction ultra-structural morphology. Thus, by acting as a Core, we will provide essential cell supplies, much needed technical expertise in NO detection and immunocytochemistry, TEM, and quality assurance, so contributing to the ability to directly compare data from different projects and between cell types.