Tissue/Pathology Core

该组织/病理学核心为参与宫颈癌孢子和其他合作研究工作的研究人员提供了具有良好特征的人类生物标本。在第一个资助期，该中心从子宫颈癌孢子中心登记的3524名患者中收集了超过78000份等份样本，到目前为止分发了6332份等份样本。这包括迄今来自838名患者的28,000多份等量样本，这些样本来自JHU乳腺癌和头颈部孢子的两项流行病学研究，而且不需要额外费用。除了日益增长的复杂样品采集需求外，我们的 研究人员依靠专家的病理学支持来确保所选研究的适当组织准备和特征。该中心提供此类专家对标本的病理评估和技术支持。具体地说，这一核心继续：1.在不影响患者护理或保密的情况下，获得知情同意并收集患者的样本用于转译研究；2.收集宫颈癌和癌前病变以及患者的正常组织，包括那些为孢子项目登记的临床试验的组织；3.收集患者的血液、分泌物和脱落细胞(例如宫颈刮片)，包括为孢子项目登记的临床试验；4.按照SOP处理和存储临床标本，以满足所有孢子调查人员的要求；5.将标本信息输入到 中央数据库系统和跟踪标本的分配和运输；6.根据来源、病理分级和分期以及肿瘤和间质组织的比例确定组织标本的特征；7.使用明确的机制，优先将所请求的标本分配给调查人员 约翰霍普金斯孢子内外；8.以尽可能廉价和有效的方式及时提供有质量控制的标本；9.运送标本进行组织学和病毒学分析，例如免疫组织化学染色、原位杂交、组织微阵列、HPV检测和分型、免疫表型鉴定或收费综合癌症中心核心的激光捕获显微解剖 设施；10.支持开发和实施免疫学检测。样本将使用CaTIssue进行跟踪，这是一个CaBIG投诉数据库，可供我们在UAB和UC的项目访问。该核心的活动将与行政/临床核心A、生物统计和数据管理核心B和免疫学核心D的活动相结合，以确保标本和临床信息得到适当编目和传播。

This Tissue/Pathology Core provides well-characterized human biological specimens to researchers participating in this Cervical Cancer SPORE and other collaborative research efforts. In the first funding period this core collected over 78,000 individual aliquots from over 3524 patients enrolled by the SPORE in Cervical Cancer and distributed 6332 aliquots to date. This includes over 28,000 aliquots from 838 patients to date from a clinical trial and two epidemiologic studies of the JHU Breast Cancer and Head and Neck SPOREs at no additional cost. In addition to the growing need for sophisticated sample acquisition, our investigators depend on expert pathology support to ensure proper tissue preparation and characterization for selected studies. This Core provides such expert pathologic evaluation of specimens and technical support. Specifically, this core continues to: 1. Obtain informed consent and collect specimens from patients for translational research without compromise of patient care or confidentiality; 2. Collect cervical carcinoma and pre-malignant lesions, as well as normal tissue from patients, including those enrolled in clinical trials for the SPORE projects; 3. Collect blood, secretions and exfoliated cells (e.g. cervical scrapes) from patients, including those enrolled in clinical trials for the SPORE projects; 4. Process and store clinical specimens following SOPs to address the requirements of all SPORE investigators; 5. Input specimen information into central database system and track specimen distribution and transport; 6. Characterize tissue specimens with respect to site of origin, pathologic grading and staging, and proportion of neoplastic and stromal tissue; 7. Use well-defined mechanisms for prioritization of the distribution of requested specimens to investigators within and external to the Johns Hopkins SPORE; 8. Provide quality-controlled specimens in a timely fashion as inexpensively and efficiently as possible; 9. Route specimens for histologic and virologic analyses e.g. immunohistochemical staining, in situ hybridization, tissue microarraying, and HPV testing and typing, immunophenotyping, or laser-capture microdissection in fee-per-service Comprehensive Cancer Center core facilities; 10. Support the development and implementation of immunologic assays. The samples will be tracked using CaTissue, a CaBIG complaint database, web-enabled for access by our projects at UAB and UC. The activities of this Core will be integrated with those of the Administrative/Clinical Core A, the Biostatistics and Data Management Core B and the Immunology Core D to ensure that specimens and clinical information are appropriately catalogued and disseminated.