LSR-Fortessa upgrade for Shared Resource

描述（由申请人提供）：本申请申请为Lombardi综合癌症中心（LCCC）购买一台Becton Dickinson LSR-Fortessa流式细胞仪。该仪器将提高该资源的能力和容量，该资源拥有乔治城大学唯一的流式细胞仪。提议的新细胞仪将允许更复杂的多色（多达13个荧光参数）分析需要正在进行的NIH资助的项目在大学。拟议的仪器将允许共享资源提供不间断的分选服务，乔治城大学医学中心的这一额外的多激光分析仪。详细介绍了共享资源的10个主要用户的研究项目。LCCC流式细胞术/细胞分选共享资源维护着乔治城大学唯一的荧光激活细胞分选器（FACS）。这一资源被来自医学院和本科校园的研究人员大量利用。2011年，46名研究人员利用该设施进行了1200多项实验。目前，有超过35名研究人员与同行评审资助的项目利用该设施。共享资源成立于1988年，由癌症中心资助、NCI CCSG拨款支持和用户费用共同支持。一个咨询委员会监督费用、政策，并为未来的增长提出建议。

DESCRIPTION (provided by applicant): This proposal requests the funds to purchase a Becton Dickinson LSR-Fortessa flow cytometer for the Lombardi Comprehensive Cancer Center (LCCC) Flow Cytometry/Cell Sorting Shared Resource. This instrument will enhance the capabilities and capacity of the resource, which houses the only flow cytometers at Georgetown University. The proposed new cytometer will allow more complex multicolor (up to thirteen fluorescence parameters) analysis required for ongoing NIH funded projects in the University. The proposed instrument will allow the Shared Resource to provide uninterrupted sorting service to the Georgetown University Medical Center by the additional of this multilaser analyzer. Research projects from 10 major users of the Shared Resource are detailed. The LCCC Flow Cytometry/Cell Sorting Shared Resource maintains the only fluorescence activated cell sorter (FACS) at Georgetown University. This resource is heavily utilized by investigators from departments in the medical school and undergraduate campus. In 2011, 46 investigators utilized the facility for more than 1200 experiments. Currently, there are more than 35 investigators with peer- reviewed funded projects utilizing the facility. The Shared Resource, established in 1988, is supported by a combination of Cancer Center funding, NCI CCSG grant support, and user fees. An advisory committee oversees fees, policies, and makes recommendations for future growth.