Remote focusing through spatial light modulation for multifocal multiphoton micro

描述(由申请人提供)：非线性光学显微镜技术(例如双光子荧光)正被用于获取探测数百微米的散射组织的体积(即三维)图像。这些技术正在与快速采集方案相结合，以允许在高NA下对活的、移动的标本进行成像。这种组合允许对生物标本进行高分辨率的活体探索。然而，增加的系统复杂性阻碍了这些显微技术很容易被生物和医学界利用，而这些社区将从这些技术中受益匪浅。因此，我们建议开发一种简化的共焦显微镜结构，它可以快速获取散射介质中的体积图像。它使用经济高效且用户友好的组件，这意味着它们不需要光学、电气或机械专业知识来设置和操作。

DESCRIPTION (provided by applicant): Nonlinear optical microscopy techniques (such as two-photon florescence) are being used to acquire volumetric (i.e. three dimensional) images that probe several hundred microns into scattering tissue. These techniques are being combined with fast acquisition schemes to allow imaging of live, moving specimens at high NA. This combination allows for high-resolution, in vivo exploration of biological specimens. However, the added system complexity prevents these microcopy techniques from being readily exploited by the biological and medical communities that would greatly benefit from them. Therefore, we propose the development of a simplified confocal microscope configuration that rapidly acquires volumetric images within a scattering media. It uses cost-effective and user-friendly components, meaning they do not require optical, electrical or mechanical expertise to setup and operate.