MICROSCOPY CORE

显微镜、图像分析和体视学核心位于BBSRB四楼，提供神经创伤和其他神经科学研究所需的先进仪器，包括两台奥林巴斯Provis AX70荧光显微镜(通过MagnaFire数码相机接口的Image Pro)、一台奥林巴斯BX 41摄像机、一台奥林巴斯BX 50荧光显微镜(BioQuant立体组件+与MagnaFire数码相机和光电摄像机接口的3D地形仪)、 蔡司Axiovert 200M显微镜和带有DAGE摄像机的尼康E400教学显微镜。除了现有的设备外，SCoBIRC最近还在美国国立卫生研究院的资助下投资了三台新的专业显微镜。第一种是奥林巴斯FluoViewTM 300点扫描、点检测、共焦激光扫描显微镜，专为生物研究应用而设计，具有优异的分辨率、效率和 励磁，以及直观、用户友好的界面，包括为个人用户预设的参数。FluoViewTM 300还能够进行3D渲染和延时观察，并可生成TTL I/O信号以协调与外部仪器计时的实验。此外，两个高灵敏度光电倍增管(PMT)直接位于FV300共焦荧光发射光路内，用于高灵敏度检测荧光信号。第二个体视学显微镜是倒置的 奥林巴斯IX 81配备了电动舞台编码器和BioQuant Nova Prime软件，特别适合使用现代体视学工具进行量化。中心由一名核心主任Patrick Sullivan博士、SCoBIRC副主任兼解剖学和神经生物学副教授和一名助理主任Alexander Rabchevsky博士和一名生理学副教授Alexander Rabchevsky组成，两人都很好 精通在研究中使用多种图像分析、终点测量和共聚焦成像作为终点。两人都拥有开发用于评估神经创伤研究中组织保留和细胞丢失的方案的专业知识。

The Microscopy, Image Analysis and Stereology Core, located on the BBSRB fourth floor, provides advanced instrumentation required for neurotrauma and other neuroscience research, including two Olympus Provis AX70 Fluorescence Microscopes (Image Pro interfaced via MagnaFire Digital Camera), an Olympus BX 41 with Video Camera, an Olympus BX 50 Epifluorescent Microscope (Bioquant Stereology package + 3D topographer interfaced with MagnaFire Digital Camera and Optronics Video Camera), a Zeiss Axiovert 200 M microscope, and a Nikon E400 teaching microscope with DAGE Video Camera. In addition to this existing equipment, SCoBIRC recently invested in three new specialized microscopes with funding from the NIH. The first, an Olympus FluoViewTM 300 point-scanning, point-detection, confocal laser scanning microscope designed for biology research applications, has excellent resolution, efficiency of excitation, and an intuitive, user-friendly interface including preset parameters for individual users. The FluoViewTM 300 is also capable of 3D rendering and time-lapse observation, and TTL I/O signals can be generated to coordinate experiments timed with external instrumentation. Additionally, two high-sensitivity photomultiplier tubes (PMTs) are located directly within the FV300 confocal fluorescence emission light path for high sensitivity detection of the fluorescence signal. The second stereology microscope is an inverted Olympus IX 81 outfitted with motorized stage encoders and the BioQuant Nova Prime software, particulariy well suited for quantification using modern stereological tools. The core is staffed by a Core Director, Patrick Sullivan, Ph.D., SCoBIRC Associate Director and Associate Professor of Anatomy & Neurobiology and an Assistant Director, Alexander Rabchevsky, Ph.D., Associate Professor of Physiology both of whom are well versed in the use of multiple image analysis endpoint measures and confocal imaging as endpoints in theirresearch. Both have expertise in developing protocols used to assess tissue sparing and cell loss in neurotrauma research.