CCR-Frederick Flow Cytometry Core

CCR-Flow Core是Frdereick国家癌症研究实验室的NCI和承包商研究人员不可或缺的资源。来自35个不同实验室的70名研究人员利用了流动核心工作人员的专业知识以及工作人员提供和维护的仪器。研究人员一直在研究的项目包括肿瘤微环境中高亲和力T细胞的耐受性。另一项研究是肿瘤坏死因子在稳定CD 4 + Foxp 3+调节性T细胞中的关键作用。还研究了胰腺癌干细胞的NF-B信号通路的调节。还完成了一个研究IKK α在自发性肺鳞状细胞癌中的关键作用的项目。HLA-C表达对艾滋病毒控制的影响也是一个研究项目。DC细胞在急性感染性炎症过程中的作用已被研究。在今年的前三个季度，流动核心工作人员已经培训了18名调查人员，让他们运行自己的样本。受过训练的调查员每天使用这些仪器，经常使用到深夜和周末。这就给流量核心人员留出了更多的时间，在今年前三个季度对约450个样本进行分类，对约10419个样本进行分析。如果没有调查人员获取数据和分析自己的样本，政府将不得不雇用全职经验丰富的个人来完成同样数量的工作。在过去一年中，至少发表了15篇论文，8篇论文正在印刷中，14篇论文是利用流动核心获得的数据提交的。未来的目标包括通过将经过培训的调查人员队伍扩大到弗雷德里克的所有CCR，从而扩大使用核心仪器和专业知识产生的科学的使用，生产力和质量，从而跟上流动实验室的尖端技术。有必要对所有仪器进行升级，使激光和荧光染料功能更具互换性，以便研究者能够灵活使用多台仪器。这将防止数据丢失，因为所需的仪器正在由另一名研究人员使用或因需要维修而停机。在样品分析以及细胞分选中具有这种灵活性至关重要。我们还希望保持先进的设施，这将需要至少一个分析仪升级到一次分析至少28个参数。

The CCR-Flow Core is an indispensable resource for NCI and contractor investigators at the Frdereick National Lab for Cancer research. Seventy investigators from 35 different laboratories have used the expertise of the flow core staff and the instrumentation available and maintained by the staff. The projects investigators have been studying include tolerization of the high-avidity T cells in the tumor microenvironment. Another study was of the critical role of tumor necrosis factor in stabilizing the CD4+Foxp3+ regulatory T cell. Regulation of the NF-B signaling pathway of pancreatic cancer stem cells was also studied. A project looking at the pivotal role of IKKalpha in spontaneous lung squamous carcinomas was also done. The influence of HLA-C expression on HIV control was also a project of study. Studies have been done on the role of DC cells in the acute infectious inflammatory process. The flow core staff has trained eighteen investigators in the first three quarters of the year to run their own samples. The instruments are used daily by the trained investigators often into the night and on the weekends. This leaves more time for the flow core staff to sort approximately 450 samples and analyze about 10419 samples in the first three quarters of this year. Without the investigators acquiring the data and analyzing their own samples, the government would have to hire full time experienced individuals to perform the same volume of work. At least 15 papers have been published, 8 papers are in press and 14 papers have been submitted in the past year using data obtained in the flow core. The future goals include keeping up with cutting-edge technology in the flow lab by expanding the pool of investigators trained to run and analyze their own samples to all of CCR in Frederick thereby expanding the use, productivity and quality of the science generated using the core's instrumentation and expertise. It is necessary to up-grade all of the instruments so that laser and fluorochrome capabilities are more interchangeable so that investigators have the flexability to use more than one instrument. This would prevent loss of data because the instrument needed was in use by another investigator or down because of needed repairs. It is critical to have this flexability in analysis of samples as well as for sorting of cells. We would also like to stay a cutting edge facility which would require at least one analyzer to be up-graded to analyze at least 28 parameters at once.