Neuroanatomy Core - Developmental Exposure Alcohol Research Center

神经解剖学的核心是每一个主要项目的组成部分.IT协作 与解除弧线成员就各种科学问题和方法进行磋商。核心提供特定的专业知识 关于神经病理学方法的组织，以分析与神经可塑性相关的神经元可塑性 产前和青少年接触乙醇。具体目标如下：(一)做好核心人事工作 了解细胞、层和核结构的结构/功能，以及 发育中(如胎儿和青少年)啮齿动物大脑中的联系；(2)与钥匙 对于特定项目的人员，核心负责纸巾的准备工作。探索大脑的方法 结构包括组织学方法和免疫组织化学方法。代谢研究依赖于2-脱氧-D葡萄糖 放射自显影和CFOS免疫组织化学结合免疫组织化学 方法聚焦于特定的神经元表型；(3)光学和共聚焦显微镜和数字成像将 用于用显微密度法、体视学和颗粒计数方法对结果进行量化； 以及(4)数字图像处理将配备实时显微视频会议系统，用于 对方法和调查结果进行集体评估，以及另一个制作插图以供出版的系统。 因此，神经解剖学核心在基础科学和方法方面提供了宝贵的专业知识。 有必要进行所有的主要项目和可能的试点项目。 演出现场(S)(组织、市、州) 美国国务院

The NEUROANATOMY CORE is and integral part of every Main Project in the DEARC. It collaborates with DEARC members on a variety of scientific issues and methods. The CORE provides specific expertise on the organization of neuropathological methods for analyzing neuronal plasticity associated with the prenatal and adolescent exposure to ethanol. The Specific Aims are as follows: (1) CORE personnel work with DEARC members to understand the structure/function of cell, layer, and nuclear architecture, and connections in the developing (e.g., fetal and adolescent) rodent brain; (2) in conjunction with the key personnel for a particular project, the CORE is responsible for tissue preparation. Methods to explore brain structure include histological methods and immunohistochemistry. Metabolic studies rely on 2-deoxy-Dglucose autoradiography and cFos immunohistochemistry which are paired with immunohistochemical methods to focus on specific neuronal phenotypes; (3) light and confocal microscopy and digital imaging will be provided for quantification of findings with microdensitometry, stereology, and grain counting methods; and (4) digital image processing will be provided with a live microscopic, video-conferencing system for group evaluation of methods and findings and another system for producing illustrations for publication. Thus, the NEUROANATOMY CORE provides invaluable expertise in the basic science and methods necessary to conduct all of the Main Projects and may of the Pilot Projects. PERFORMANCE SITE(S) (organization, city, state) Department of