NEURONAL CONNECTIONS AND RESPONSES IN CINGULATE CORTEX

前扣带皮层与情感 对伤害性刺激的反应含有阿片样物质合成神经元 并具有高密度的μ和δ阿片受体。 不 已知哪些神经过程结合阿片受体配体。 的 本实验将采用实验定位 私家侦探发明的技术用于定位轴突， 树突状毒蕈碱乙酰胆碱受体亚型。 用于全 切片分析将在大鼠中进行三种类型的病变之一 脑，随后测定μ和δ-选择性配体结合 在冷冻切片机切片中，盖玻片放射自显影和单个 前扣带回皮质颗粒计数：1）全深度或 板层鹅膏蕈氨酸，2）选择性破坏皮质 投射神经元注射免疫毒素OX 7-ss- 皂草素进入尾状核或导水管周围灰质核或进入 后扣带回皮质; 3）去传入神经根性损害。 第二种定位方法将涉及尾状核的预标记， 中脑导水管周围灰质或皮质投射神经元具有稳定， 逆行输送荧光染料。 皮层神经元将 然后酶促和机械解离并测定 β-呋喃甲胺与预先标记的锥体细胞的μ-选择性结合 神经元和未标记的锥体和多极神经元。 另一 一系列的实验将评估皮层中的mu和delta位点， 在冰冻切片中， 兔子和人脑 由于丘脑和皮层的数量 连接可能涉及感觉运动方面， 对伤害性刺激的反应，多标签连接研究是 还建议分析丘脑皮质和皮质 家兔扣带皮层的连接。 对丘脑的研究将 强调外侧大细胞核，因为它的连接 没有被描述过，也不知道存在于任何其他地方。 哺乳动物。 在皮层水平的研究将强调地形 扣带区之间以及扣带与视觉之间的关系 地区 这些研究的主要目的是阐明一种直接的方法， 阿片和阿片类化合物影响皮质神经元， 项目自主和躯体运动中心，表征 阿片受体在树突和胞体上的分布 锥体投射神经元和多极中间神经元， 描述了兔子体内一个几乎未知的细胞核的连接 丘脑 分离的成年皮层神经元可用于未来 研究评估阿片类药物的上调和下调机制 受体以及阿片类化合物对离子通道的门控。

Anterior cingulate cortex has been implicated in affective responses to noxious stimuli contains opioid synthesizing neurons and has high densities of mu and delta opioid receptors. It is not known which neuronal processes bind opioid receptor ligands. The present experiments will employ experimental localization techniques developed by the P.I. for localizing axonal and dendritic muscarinic acetylcholine receptor subtypes. For whole section analysis one of three types of lesions will be made in rat brain followed by assay of mu and delta-selective ligand binding in cryomicrotome sections, coverslip autoradiography and single grain counting in anterior cingulate cortex: 1) full depth or laminar ibotenic acid, 2) selective destruction of cortical projection neurons following injections of the immunotoxin OX7-ss- saporin into the caudate or periaqueductal gray nuclei or into posterior cingulate cortex, 3) deafferentation undercut lesions. A second localization approach will involve prelabeling of caudate, periaqueductal gray or cortical projection neurons with a stable, retrogradely transported fluorescent dye. Cortical neurons will then be enzymatically and mechanically dissociated and assayed for mu-selective binding of beta-funaltrexamine to prelabeled pyramidal neurons and unlabeled pyramidal and multipolar neurons. Another series of experiments will evaluate mu and delta sites in cortical sublaminae and thalamic subnuclei in cryomicrotome sections of rabbit and human brains. As a number of thalamic and cortical connections are likely involved in sensorimotor aspects of responses to noxious stimuli, multiple label connection studies are also proposed to analyze thalamocortical and corticocortical connections of rabbit cingulate cortex. Studies in thalamus will emphasize the lateral magnocellular nucleus because its connections have not been described and it is not known to exist in any other mammal. Studies at the cortical level will emphasize topographic relations among cingulate areas and among cingulate and visual areas. The main goals of these studies are to elucidate a direct means by which opiate and opioid compounds influence cortical neurons which project to autonomic and somatic motor centers, characterize the distribution of opioid receptors on the dendrites and somata of pyramidal projection neurons and multipolar interneurons and describe the connections of a virtually unknown nucleus in rabbit thalamus. Dissociated adult cortical neurons can be used in future studies to evaluate mechanisms of up and down regulation of opioid receptors as well as gating of ionic channels by opioid compounds.