NEURONAL CONNECTIONS AND RESPONSES IN CINGULATE CORTEX
NEURONAL CONNECTIONS AND RESPONSES IN CINGULATE CORTEX
批准号:
3398782
负责人:
Brent Alan Vogt
金额:
$12.87万
依托单位国家:
美国
项目类别:
财政年份:
1982
资助国家:
美国
项目状态:
已结题
起止时间:
1982-07-01 至 1990-08-31
关键词:
acetylcholine autoradiography brain mapping brain regulatory center dendrites dynorphins electron microscopy electrophysiology fluorescence human tissue interneurons laboratory rabbit laboratory rat ligands motor neurons muscarinic receptor neural information processing neuroanatomy neuropeptide receptor neurophysiology opioid receptor pain periaqueductal gray matter pyramidal cells sectioning sensorimotor system silver impregnation stimulus /response thalamic nuclei thalamocortical tract tritium
中文摘要
前扣带皮层被认为与情感有关
英文摘要
Anterior cingulate cortex has been implicated in affective
responses to noxious stimuli contains opioid synthesizing neurons
and has high densities of mu and delta opioid receptors. It is not
known which neuronal processes bind opioid receptor ligands. The
present experiments will employ experimental localization
techniques developed by the P.I. for localizing axonal and
dendritic muscarinic acetylcholine receptor subtypes. For whole
section analysis one of three types of lesions will be made in rat
brain followed by assay of mu and delta-selective ligand binding
in cryomicrotome sections, coverslip autoradiography and single
grain counting in anterior cingulate cortex: 1) full depth or
laminar ibotenic acid, 2) selective destruction of cortical
projection neurons following injections of the immunotoxin OX7-ss-
saporin into the caudate or periaqueductal gray nuclei or into
posterior cingulate cortex, 3) deafferentation undercut lesions.
A second localization approach will involve prelabeling of caudate,
periaqueductal gray or cortical projection neurons with a stable,
retrogradely transported fluorescent dye. Cortical neurons will
then be enzymatically and mechanically dissociated and assayed for
mu-selective binding of beta-funaltrexamine to prelabeled pyramidal
neurons and unlabeled pyramidal and multipolar neurons. Another
series of experiments will evaluate mu and delta sites in cortical
sublaminae and thalamic subnuclei in cryomicrotome sections of
rabbit and human brains. As a number of thalamic and cortical
connections are likely involved in sensorimotor aspects of
responses to noxious stimuli, multiple label connection studies are
also proposed to analyze thalamocortical and corticocortical
connections of rabbit cingulate cortex. Studies in thalamus will
emphasize the lateral magnocellular nucleus because its connections
have not been described and it is not known to exist in any other
mammal. Studies at the cortical level will emphasize topographic
relations among cingulate areas and among cingulate and visual
areas.
The main goals of these studies are to elucidate a direct means by
which opiate and opioid compounds influence cortical neurons which
project to autonomic and somatic motor centers, characterize the
distribution of opioid receptors on the dendrites and somata of
pyramidal projection neurons and multipolar interneurons and
describe the connections of a virtually unknown nucleus in rabbit
thalamus. Dissociated adult cortical neurons can be used in future
studies to evaluate mechanisms of up and down regulation of opioid
receptors as well as gating of ionic channels by opioid compounds.
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NeuroAnatomy Core
-
批准号:7555167
-
项目类别:
-
资助金额:$28.59万
-
财政年份:2009
-
负责人:Brent Alan Vogt
-
依托单位:
Medial Pain Inputs to Monkey Anterior Cingulate Cortex
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批准号:6720944
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项目类别:
-
资助金额:$28.12万
-
财政年份:2003
-
负责人:Brent Alan Vogt
-
依托单位:
Medial Pain Inputs to Monkey Anterior Cingulate Cortex
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批准号:6984755
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项目类别:
-
资助金额:$27.46万
-
财政年份:2003
-
负责人:Brent Alan Vogt
-
依托单位:
Medial Pain Inputs to Monkey Anterior Cingulate Cortex
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批准号:6830847
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项目类别:
-
资助金额:$28.12万
-
财政年份:2003
-
负责人:Brent Alan Vogt
-
依托单位:
Medial Pain Inputs to Monkey Anterior Cingulate Cortex
-
批准号:7153550
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项目类别:
-
资助金额:$26.66万
-
财政年份:2003
-
负责人:Brent Alan Vogt
-
依托单位:
Medial Pain Inputs to Monkey Anterior Cingulate Cortex
-
批准号:7340374
-
项目类别:
-
资助金额:$26.66万
-
财政年份:2003
-
负责人:Brent Alan Vogt
-
依托单位:
MULTIFOCAL NEURODEGENERATION IN ALZHEIMER'S DISEASE
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批准号:6082539
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项目类别:
-
资助金额:$25.77万
-
财政年份:2000
-
负责人:Brent Alan Vogt
-
依托单位:
MULTIFOCAL NEURODEGENERATION IN ALZHEIMER'S DISEASE
-
批准号:6363935
-
项目类别:
-
资助金额:$16.97万
-
财政年份:2000
-
负责人:Brent Alan Vogt
-
依托单位:
MULTIFOCAL NEURODEGENERATION IN ALZHEIMER'S DISEASE
-
批准号:6312626
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项目类别:
-
资助金额:$2.54万
-
财政年份:2000
-
负责人:Brent Alan Vogt
-
依托单位:
MULTIFOCAL NEURODEGENERATION IN ALZHEIMER'S DISEASE
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批准号:6531091
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项目类别:
-
资助金额:$26.18万
-
财政年份:2000
-
负责人:Brent Alan Vogt
-
依托单位:
MULTIFOCAL NEURODEGENERATION IN ALZHEIMER'S DISEASE
-
批准号:6547439
-
项目类别:
-
资助金额:$7.24万
-
财政年份:2000
-
负责人:Brent Alan Vogt
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依托单位:
ALZHEIMER'S DISEASE CLASSES AND CINGULATE REORGANIZATION
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批准号:3123405
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项目类别:
-
资助金额:$11.35万
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财政年份:1992
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负责人:Brent Alan Vogt
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依托单位:
ALZHEIMERS DISEASE CLASSES AND CINGULATE REORGANIZATION
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批准号:2052663
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项目类别:
-
资助金额:$11.8万
-
财政年份:1992
-
负责人:Brent Alan Vogt
-
依托单位:
ALZHEIMER'S DISEASE CLASSES AND CINGULATE REORGANIZATION
-
批准号:3123404
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项目类别:
-
资助金额:$10.67万
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财政年份:1992
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负责人:Brent Alan Vogt
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依托单位:
NEURONAL CONNECTIONS AND RESPONSES IN CINGULATE CORTEX
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批准号:3398781
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项目类别:
-
资助金额:$10.1万
-
财政年份:1982
-
负责人:Brent Alan Vogt
-
依托单位:
NEURONAL CONNECTIONS AND RESPONSES IN CINGULATE CORTEX
-
批准号:3398780
-
项目类别:
-
资助金额:$8.85万
-
财政年份:1982
-
负责人:Brent Alan Vogt
-
依托单位:
NEURONAL CONNECTIONS AND RESPONSES IN CINGULATE CORTEX
-
批准号:3398783
-
项目类别:
-
资助金额:$11.4万
-
财政年份:1982
-
负责人:Brent Alan Vogt
-
依托单位:
NEURONAL CONNECTIONS & RESPONSES IN CINGULATE CORTEX
-
批准号:3398777
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项目类别:
-
资助金额:$12.64万
-
财政年份:1982
-
负责人:Brent Alan Vogt
-
依托单位:
NEURONAL CONNECTIONS AND RESPONSES IN CINGULATE CORTEX
-
批准号:3398775
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项目类别:
-
资助金额:$10.48万
-
财政年份:1982
-
负责人:Brent Alan Vogt
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依托单位:
Neuroanatomy Core - Developmental Exposure Alcohol Research Center
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批准号:8537095
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项目类别:
-
资助金额:$6.73万
-
财政年份:--
-
负责人:Brent Alan Vogt
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依托单位:
海外基金