A Functionalized Nanoparticle-Based handheld Device for Rapid and Sensitive Detec

产品说明：有机磷酸酯（OP）农药是在许多农业和园林应用中用于控制昆虫种群的高毒性化合物。这些有毒化学品的广泛使用已造成严重的环境健康风险。敏感、准确地进行有机磷农药环境暴露的生物监测和健康风险评价是至关重要的。然而，简单，快速和定量的诊断技术和设备检测环境OP暴露还没有。这个小企业创新研究项目是开发一种手持式生物监测设备，包括功能化纳米颗粒和侧流测试条，用于使用血液样本对OP的环境暴露进行简单，快速，具有成本效益和定量检测。该项目利用磷酸化胆碱酯酶（OP-ChE）作为OP暴露的生物标志物。在第一阶段，有三个具体目标：（1）开发结合OP-ChE的Zr（IV）功能化荧光纳米颗粒（Zr-FFNP）。(2)开发一种基于Zr-FFNP的新型OP-ChE免疫分析方法。(3)将基于Zr-FFNP的免疫测定调整为用于检测OP-ChE的侧向流测试条系统。该研究将确定检测限，响应时间，动态范围和其他关键性能指标的设备使用血液样本在体外剂量与三种典型的农药，并证明了手持式生物监测设备的可行性，灵敏地检测OP暴露。第二阶段将进一步开发一种专门用于检测有机磷农药暴露的手持式生物监测装置。该装置将通过使用多种有机磷农药的体外血液，以及从农场附近学校的学生和涉及使用有机磷农药的工人的家庭成员的血液样本进行验证。根据该计划开发的便携式生物监测设备将提供一种即时工具，用于快速，灵敏，具有成本效益和实时检测环境暴露于OP农药。

DESCRIPTION: Organophosphate (OP) pesticides are highly toxic compounds used to control insect populations in a number of agricultural and landscaping applications. The widespread use of these toxic chemicals has generated serious environmental health risks. It is vital to sensitively and accurately bio monitoring of environmental exposure to OP pesticides and assessment of its health risk. However, simple, rapid, and quantitative diagnostic technologies and devices for detecting environmental OP exposure are not available. This Small Business Innovation Research Project is to develop a handheld bio monitoring device incorporating a functionalized nanoparticle and a lateral flow test strip for simple, rapid, cost-effective, and quantitative detection of environmental exposure to OP using a blood sample. This project takes advantage of phosphorylated cholinesterase (OP-ChE) as a biomarker of OP exposure. In phase I, there are three specific aims: (1) Develop Zr (IV)-functionalized fluorescence nanoparticles (Zr-FFNPs) that bind to OP-ChE. (2) Develop a Zr-FFNP-based new immunoassay for detecting OP-ChE. (3) Adapt the Zr-FFNP-based immunoassay to a lateral flow test strip system for detecting OP-ChE. The research will determine the detection limits, response time, dynamic range, and other key performance metrics of the device using blood samples in vitro dosed with three typical pesticides and prove the feasibility of the handheld bio monitoring device for sensitively detecting OP exposure. Phase II will further develop a specialized hand-held bio monitoring device for detecting exposure to OP. The device will be validated with blood in vitro dosed with a wide range of OP pesticides and blood samples from students in schools near farms and family members of workers who involved in the used of OP pesticides. The portable biomonitoring device developed under this program will provide a point-of-care tool for rapid, sensitive, cost-effective, and real-time detection of environmental exposure to OP pesticides.