Small Animal Imaging

项目总结(见说明)： 基于分子的成像技术为评估潜在治疗剂的药代动力学和靶向特性以及评估体内重要的细胞过程提供了独特的机会。通过非侵入性成像监测癌症分子过程的能力可能提供有关治疗效果的关键信息。在临床前研究的背景下，使用活体成像可以获得单个动物上分子示踪剂的完整动态生物分布研究，从而减少了达到统计上适当结果所需的动物数量。通常，在小动物成像模式中测试的成像技术和靶向试剂可以直接转移到临床环境中。小动物成像核心(SAIC)是一个共享资源，致力于为研究人员提供最先进的小动物成像功能，用于与希望之城癌症中心的使命相关的基础和翻译研究。SAIC的具体目标包括：(1)与癌症研究相关的小动物成像的最新发展、当前能力和局限性；(2)在小动物成像实验室的背景下实施、开发、校准、维护和操作相关成像系统；以及(3)与研究人员合作，在希望之城的研究中优化小动物成像的使用。目前核心人员包括一名主任、一名成像物理学家和一名经理，他们都在利用成像进行动物研究方面经验丰富。运行中的小动物成像系统包括两个用于生物发光光学成像的装置(一个已改装用于荧光成像[IVIS 100，卡利珀生命科学公司])；一个伽马相机(y-Imager，BiSpace，Inc.)；一个PET扫描仪(microPET R4，西门子)；和一个CT扫描仪(microCAT II Hi Res，西门子)。MicroPET和microCAT很容易串联使用，以生成共同配准的功能解剖PET/CT图像。动物资源中心在帕文生物医学研究大楼内提供了三个房间供SAIC使用(一个房间用于microPET、microCAT和y-Imager，两个房间用于IVIS光学成像仪器。已经开发了一种系统，用于监测仪器的使用情况，并向用户收取成像程序的部分费用。

PROJECT SUMMARY (See instructions): Molecular-based imaging provides unique opportunities to assess the pharmacokinetics and targeting properties of r potential therapeutic agents, as well as to assess vital cellular processes in vivo. The ability to monitor the molecular processes of cancer through non-invasive imaging may provide critical information regarding the effects of therapy. In the context of pre-clinical research, the use of in vivo imaging permits the acquisition of a complete dynamic biodistribution study of a molecular tracer on an single animal, thereby reducing the number of animals required to reach a statistically adequate result. Often the imaging techniques and targeting agents that are tested in small animal imaging modalities are directly transferable to the clinical setting. The Small Animal Imaging Core (SAIC) is a shared resource dedicated to providing investigators access to state of the art small animal imaging capabilities for use in basic and translational research relevant to the mission of the City of Hope Cancer Center. Specific aims of the SAIC include: (1) keeping abreast of the latest developments, current capabilities, and limitations of small animal imaging as pertains to cancer research; (2) implementing, developing, calibrating, maintaining, and operating relevant imaging systems within the context of a small animal imaging laboratory; and (3) optimizing the use of small animal imaging in research at City of Hope in collaboration with investigators. Core personnel currently include a Director, an imaging physicist, and a manager, all of whom are highly experienced in the use of imaging for research with animals. Small animal imaging systems in operation include two units for bioluminescence optical imaging (one has been modified for fluorescence imaging [IVIS 100, Caliper Life Sciences]); a gamma camera (y- IMAGER, Biospace, Inc.); a PET scanner (microPET R4, Siemens); and a CT scanner (microCAT II Hi Res, Siemens). The microPET and microCAT are readily used in tandem to generate co-registered functional anatomic PET/CT images. The Animal Resources Center has provided three rooms within the Parvin Biomedical Research Building for use by the SAIC (one room for the microPET, microCAT, and the y- IMAGER, and two rooms for the IVIS optical imaging instruments. A system has been developed for monitoring instrument usage and to bill users for a portion of the costs of the imaging procedures.