Mass Spectrometry and Proteomics Shared Resouce

质谱和蛋白质组学（MSP）共享资源为亨斯迈癌症研究所（HCI）成员和整个犹他大学研究界提供广泛的质谱服务，包括最先进的仪器，咨询和专业知识，特别是基于蛋白质组学的研究。混合傅立叶变换质谱仪（LTQ-FT，热电子）是主要的蛋白质组学仪器。它提供低至50原子摩尔水平的蛋白质鉴定（ID），高质量精度（<2ppm），并通过电子捕获解离（ECD），红外多光子解离（IRMPD）和碰撞诱导解离（CID）进行肽测序。蛋白质组学服务包括蛋白质ID；翻译后修饰的表征（如磷酸化）；以及完整蛋白质的分析。该资源还为广泛的其他研究应用提供质谱服务，如天然产物、药物和药物-聚合物偶联物、核酸、DNA扩增产物（如PCR）、大生物分子和聚合物、肽和小合成分子的结构分析。查德·纳尔逊博士自2003年起在两名工作人员的协助下指导MSP共享资源。MSP共享资源的工具和人员集中部署。MSP共享资源服务于所有基于HCI实验室的科学项目（细胞生长和分化的核控制；细胞反应和调节；成像、诊断和治疗；胃肠道癌症），描述在癌症机制中发挥作用的分子，特别是蛋白质和翻译后修饰。此外，MSP共享资源对小化合物的表征，包括天然产物和药物传递聚合物，对HCI的发育治疗活性有重要贡献。MSP共享资源是由大学和HCI领导共同监督的机构管理设施。有一个教师咨询委员会，并通过年度调查对资源的用户满意度进行审查。该设施是按服务收费的方式运作的（退款）。2008年，MSP共享资源的总体使用率是癌症中心成员与同行评审基金和癌症中心共享资源的31%。要求CCSG提供资金以支付拟议资源预算的14%（$44 857）。MSP共享资源有足够的容量供癌症中心成员额外使用。

The Mass Spectrometry and Proteomics (MSP) Shared Resource provides Huntsman Cancer Institute (HCI) members and the entire University of Utah research community a broad range of mass spectrometry services, including state-of-the-art instrumentation, consultation, and expertise, particularly for proteomics-based research. The hybrid Fourier-Transform mass spectrometer (model LTQ-FT, ThermoElectron) is the main proteomics instrument. It provides protein identification (ID) at levels as low as 50 attomole, high-mass accuracy (<2ppm), and peptide sequencing by electron-capture dissociation (ECD), infrared multiphoton dissociation (IRMPD), and collision-induced dissociation (CID). Proteomics services include protein ID; characterization of post-translational modifications (e.g., phosphorylafion); and analysis of intact proteins. The Resource also provides mass spectrometry services for a wide range of other research applications such as structural analysis of natural products, drugs and drug-polymer conjugates, nucleic acids, DNA amplification products (e.g., PCR), large biomolecules and polymers, peptides, and small synthetic molecules. Chad Nelson, PhD, has directed the MSP Shared Resource since 2003, with the assistance of two staff members. The instrumentation and staff of the MSP Shared Resource are centrally located. The MSP Shared Resource serves all HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation; Cell Response and Regulation; Imaging, Diagnostics, and Therapeutics; and Gastrointestinal Cancers), characterizing molecules, particularly proteins and post-translational modifications, that play roles in cancer mechanisms. In addition, characterization of small compounds by the MSP Shared Resource, including natural products and drug-delivery polymers, contributes significantly to the developmental therapeutics activities of HCI. The MSP Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). In 2008, overall use of the MSP Shared Resource by Cancer Center members with peer-reviewed funding and Cancer Center Shared Resources was 31 percent. Funds are requested from the CCSG to cover 14 percent ($44,857) of the proposed Resource budget. The MSP Shared Resource has ample capacity for additional use by Cancer Center members.