CAP:Cationic DNA Liposome^Microbial Complexes as Broad Spectrum Antimicrobials

我们首先确定了MPF的活性成分是热稳定的和抗蛋白酶的。 这强烈表明该组分本质上是脂质或糖。 我们接下来优化了从F.在我们的CLDC制剂中进行测试。 我们证实，与MPF类似，在不存在CLDC的情况下，脂质制剂和糖都不能激发针对F的保护性免疫应答。宿主细胞中的土拉菌。 我们接下来将脂质制剂与CLDC组合以确定它们对F.土拉菌感染 然而，作为无机分子，脂质重悬于乙醇中。 我们发现乙醇对CLDC脂质体的保留有深刻的负面影响。

We first determined that the active component of MPF was heat stable and proteinase resistant. This strongly suggested that the component was either lipid or sugar in nature. We next optimized techniques to isolate lipids and sugars from F. tularensis for testing in our CLDC preparations. We confirmed, similarly to MPF, that in the absence of CLDC neither the lipid preparations nor the sugars were able to provoke protective immune responses directed against F. tularensis in host cells. We next combined the lipid preparations with CLDC to determine their efficacy against F. tularensis infection. However, as inorganic molecules the lipids are resuspended in ethanol. We found that ethanol had a profoundly negative effect on the retention of CLDC liposomes.