MOUSE EXPERIMENTAL CORE

小鼠实验核心（MEC）设施在结肠癌研究中心（CCCR）的I期和11期期间对各种研究项目的成功至关重要，并将在111期及以后继续这样做。其目标是为南卡罗来纳州（USC）的CCCR研究人员和USC以外的研究人员提供动物研究支持。MEC已经发展成为一个核心设施，以满足投资者的独特研究需求。其具体目标如下：1）提供育种服务，支持CCCR和南加州大学研究人员的研究项目。MEC人员将对小鼠进行繁殖和基因分型，使其断奶以适应特定的饮食或实验条件，并根据需要将其交付，以促进动物实验的执行。2)在动物饲养设施内提供基础设施，以促进动物实验的执行。MEC将提供实验室空间、动物饲养室、设备和试剂，集中在靠近动物饲养室的位置，研究人员可以在那里维持他们的菌落，执行复杂的实验，并收集和分析组织标本的数据。3)提供动物实验程序和裂隙采集与分析方面的专业知识和人员培训。MEC将培训研究人员及其工作人员，并与其他核心合作举办研讨会，介绍新技术和结直肠癌和其他癌症的小鼠模型。4)在制定和执行实验方案和试点项目方面提供专门知识。基于多年的CRC遗传和正交异性小鼠模型工作经验，以及南加州大学校园内外的一群合作者，MEC将与个人研究人员合作设计和执行新项目，以促进及时获取高质量的研究数据，资助申请和出版物。MEC将建立机制，确保这一宝贵核心的长期可持续性，以便继续为南加州大学研究界和南加州大学以外的其他企业提供COBRE资助之外的宝贵服务。

PROJECT SUMMARY (See instructions): The Mouse Experimentation Core (MEC) Facility has been instrumental to the success of various research projects during Phases I and 11 of the Center for Colon Cancer Research (CCCR) and will continue to do so in Phase 111 and beyond. Its goal is to provide animal research support to the CCCR investigators of the University of South Carolina (USC) and investigators outside USC. The MEC has developed into a core facility that is responsive to the unique research needs of invesfigators. The following are its specific aims: 1) To provide breeding services in support of the research projects of the CCCR and USC investigators. The MEC personnel will breed and genotype mice, wean them to specific diets or experimental conditions, and deliver them, as needed, to facilitate the execution or animal experiments. 2) To provide the infrastructure within the animal housing facilities to facilitate the execution of animal experiments. The MEC will provide laboratory space, animal housing rooms, equipment, and reagents in a centralized location that is in close proximity to the animal housing rooms where investigators can maintain their colonies, execute complex experiments, and collect and analyze data from tissue specimens. 3) To provide expertise and personnel training on animal experimental procedures and fissure collection and analysis. The MEC will train investigators and their personnel and conduct workshops in collaboration with the other Cores to introduce new technologies and mouse models of colorectal and other cancers. 4) To provide expertise in the development and execution of experimental protocols and pilot projects. Building on years of experience working with genetic and orthotropic mouse models of CRC and on a cohort of collaborators across the USC campus and outside the university, the MEC will work with individual investigators to design and execute new projects to facilitate the timely acquisition of quality data for research, grant applications, and publications. The MEC will institute mechanisms to ensure the long term sustainability of this valuable core in order to continue to provide its valuable services to the USC research community and other enterprises outside of USC beyond the COBRE funding.