Pilot 6 - Developmental Exposure Alcohol Research Center

我们最近利用了一种新的限制获取酒精摄入量的模式，以揭示 怀孕的C57BL6/J小鼠在整个妊娠期间每天给予2小时的乙醇。孕妇每日酒精摄入量 5-6g/kg的剂量会在成年和青少年后代中产生许多行为缺陷。尤其是催眠药 对酒精的敏感性在出生后第30天和第90天降低(P&lt；0.01)，这可能表明产前 酒精暴露会改变正常发育的GABAA受体系统。这项提议的目标是 (1)确定母亲是否改变了催眠和GABAA受体对几种药物的敏感性 在受体和(2)检测不同的GABAA受体亚基在青少年和 这些老鼠的成年后代。

We have recently taken advantage of a new limited access model of binge-like ethanol intake to expose pregnant C57BL6/J mice to ethanol for 2 hours each day throughout gestation. Daily maternal ethanol intakes of 5-6 g/kg produced a number of behavioral deficits in adult and adolescent offspring. In particular, hypnotic sensitivity to ethanol was reduced at postnatal day (P) 30 and P90 (p<0.01) possibly suggesting that prenatal ethanol exposure alters the normal development GABAA receptor systems. The goals of this proposal are to (1) determine whether maternal DID alters hypnotic and GABAA receptor sensitivity to several drugs with actions at the receptor and (2) to examine the expression of various GABAA receptor subunits in the adolescent and adult offspring of these mice.