Proteomic Core

概述。蛋白质组学核心为中心，ISB和许多本地和全球合作者提供尖端的蛋白质组学能力。最近的成就包括：开发了分析和计算工具，可以对蛋白质组、亚蛋白质组和翻译后修饰进行全面和系统的分析；开发用于评估和验证蛋白质组学数据集的软件套件；设计和实施靶向定量质谱（MS）实验来分析蛋白质组和亚细胞蛋白质组。该核心配备了最先进的MS技术（请参阅参考资料），并将继续传播和推广用于高质量定量数据获取和快速实现新技术的工具。核心提供MS操作和实验设计的培训和协助，并协助蛋白质组学信息学课程（见教育和培训）。由于核心是中心研究不可或缺的一部分，我们在下面提供正在进行的技术开发的描述。

Overview. The proteomics core provides cutting-edge proteomics capabilities to the Center, ISB and many collaborators locally and worldwide. Recent accomplishments include: development of analytical and computational tools enabling comprehensive and systematic analysis of proteomes, subproteomes, and post translational modifications; development of software suites for evaluation and validation of proteomic datasets; and the design and implementation of targeted quantitative mass spectrometry (MS) experiments to analyze proteomes and subcellular proteomes. The core equipped with state-of-the-art MS technologies (see Resources) and will continue to disseminate and promote tools for high quality quantitative data acquisition and rapid implementation of new technologies. The core provides training and assistance on MS operation and experimental design, and assists with the Proteomics Informatics course (See Education and Training). Because the core is so integral to Center research, we provide below a description of ongoing technology development. Quantitative Proteomics. The core is a world leader in the development and application of both label and label-free quantitative proteomics for expression profiling and analysis of macromolecular assemblages. For example, the core, in collaboration with the Aitchison group, developed a novel automated approach to quantify peptides in SILAC experiments (QTIPs) along with new approaches for isolation of macromolecular complexes. These approaches significantly improved the identification of in vivo relevant interactions and led to extensive definition of signaling networks involved in peroxisome induction