Animal Models Core

动物模型核心的目的是为项目提供必要的慢性动物，并确保在这个PPG的各个项目中动物模型的一致性。这将消除准备工作的多变性，并将个别项目主任从创造慢性动物和照顾它们术后立即恢复的极端劳动密集型任务中解放出来。该中心还将在发生心力衰竭或假时间控制之前和期间对动物进行超声心动图评估。在过去的10年里，动物模型核心在PPG中发挥了非常有效的作用。 各种动物模型将由库尔蒂斯·G·科尼什博士制作或在其监督下制作，科尼什博士在慢性动物准备方面拥有多年经验，并在这些特定动物准备方面经验丰富。在PPG调查人员使用动物之前，他将在三名技术人员的协助下准备和照料动物。该中心将建立(1)大鼠心力衰竭(HF)冠脉结扎模型和(2)兔心衰起搏模型。 所有动物的心脏状态的评估都是通过超声心动图获得的。核心由两个超声心动图支持。兔子的成像是在Accuon Sequoia 5120机器上完成的，啮齿动物的图像是在视觉超音速Vevo 770上获得的。约翰尼·哈克利女士主要负责获取在诱发心力衰竭之前、期间和之后的大鼠和兔子的数据。

The purpose of the Animal Models Core is to provide chronic animals necessary for the projects and insure consistency in animal models across the various projects in this PPG. This will eliminate the variability of preparations and will release the individual Project Directors from the extremely labor intensive task of creating chronic animals and caring for their immediate post operative recovery. The Core will also provide echocardiographic assessment on animals prior to and during the development of heart failure or sham time controls. The Animal Models Core has worked very effectively over the past 10 years of this PPG. Various animal models will be prepared by or under the supervision of Dr. Kurtis G. Cornish who has had many years of experience in the preparation of chronic animals and is experienced in these specific animal preparations. He will be assisted by three technicians in the preparation and care of animals prior to their use by the PPG investigators. The models prepared by this Core will be (1) the coronary ligation model of heart failure (HF) in the rat, and (2) the pacing model of HF in the rabbit. Assessment of the cardiac status of all animals is obtained echocardiographically. The Core is supported by two echocardiographs. Rabbit imaging is done on an Accuson Sequoia 5120 machine and rodent images are obtained on a Visual Sonics Vevo 770. Ms. Johnnie Hackley is primarily responsible for obtaining the data on the rats and rabbits prior to, during and after the induction of heart failure.