The Effects of Lactobacillus Cell Wall in a Rat Model

尽管与许多乳杆菌属物种相关的致病作用有限，但患有严重急性胰腺炎的患者中的益生菌治疗增加了肠缺血和死亡的风险，但没有增加感染性并发症的风险（Lancet 371：651; 2008）。由于细胞壁来自革兰氏阳性菌如S。金黄色葡萄球菌可刺激全身炎症和组织损伤，我们研究了加氏乳杆菌（Lactobacillus gasseri（L. gasseri），益生菌微生物。Sprague-Dawley大鼠用5种递增剂量（10至160 mg/kg）的S. aureus或L. Gasseri细胞壁或稀释剂作为24小时输注。两种细胞壁的3种最高剂量均导致死亡，但L.加塞里与对照组相比，在输注过程中的4、8和24小时，两种细胞壁均显著增加乳酸、IL-1、IL-10、TNF、MIP-1、RANTES和一氧化氮水平，并降低循环中性粒细胞、淋巴细胞和血小板。与3种致死性S.金黄色葡萄球菌细胞壁剂量，类似L.加氏细胞壁剂量引起乳酸、IL-1、IL-10、TNF和一氧化氮的更大增加。如果将这些制剂应用于易发生肠道植物群或其产物移位的患者，则进一步确定来自益生菌的细胞壁组分的潜在致病作用似乎是重要的。 描述这项工作的手稿正在编写中。

Despite the limited pathogenic effects associated with many Lactobacillus species, probiotic treatment in patients with severe acute pancreatitis increased the risk of bowel ischemia and morality but not infectious complications (Lancet 371: 651; 2008). Since cell wall from Gram-positive bacteria like S. aureus can stimulate systemic inflammation and tissue injury, we investigated whether this could occur with cell wall from Lactobacillus gasseri (L. gasseri), a probiotic microbe. Sprague-Dawley rats were challenged with one of 5 increasing doses (10 to 160 mg/kg) of either S. aureus or L. gasseri cell wall or diluent as 24 hour infusions. The 3 highest doses of both cell walls caused mortality but this was actually greater with L. gasseri. Compared to controls, at 4, 8, and 24 hours during infusions, both cell walls significantly increased lactate, IL-1, IL-10, TNF, MIP-1, RANTES, and nitric oxide levels and decreased circulating neutrophils, lymphocytes and platelets. Compared to the 3 lethal S. aureus cell wall doses, similar L. gasseri cell wall doses caused greater increases in lactate, IL-1, IL-10, TNF, and nitric oxide. Further defining the potential pathogenic effects of cell wall components from probiotic bacteria appears important if such preparations are to be applied in patients predisposed to the translocation of intestinal flora or its products. A manuscript describing this work is in preparation.