CORE A: CELL BIOLOGY CORE

在过去的资助周期中，细胞生物学核心提供的服务的建立， 促进了与核心目标相关的各种研究。细胞生物学研究 DRTC已经扩大了研究界的出版物以及赠款证明 来自传统上不从事糖尿病和代谢领域工作的医生的申请。 DRTC的脂肪组织库和脂肪生物学专业知识和服务被转移到运营 在新资助的中大西洋营养和肥胖研究中心（NORC）的赞助下， 马里兰州大学。这确保了加强和连续性的脂肪组织相关服务， DRTC社区，同时释放资源，用于创新和扩展服务 通过巴尔的摩DRTC的细胞生物学中心细胞生物学核心启动了一个本地知识库， 组织/细胞特异性转基因CRE -缺失者脱氧土霉素可调控小鼠的转基因小鼠模型。 这些在C57 B1/6背景下的良好表征的小鼠已经成为研究C57 B1/6的有价值的资源。 研究社区。回应调查人员的需求以及与以下方面有关的相关问题： 细胞生物学在代谢和糖尿病领域扩大了其服务和专业知识， 细胞生物能量学和线粒体生物学。这一新的增加将提供专业知识和服务， 对线粒体内容物、线粒体形状和星座进行成像和定量，以评估 线粒体动力学（融合和分裂），提供线粒体基因定量方面的专业知识， 蛋白质表达（例如解偶联蛋白、电子转移链的复合物）和细胞 线粒体DNA含量。此外，DRC用户将获得独特的遗传小鼠模型（例如， floxed Drp 1，Aprieci Opal小鼠），其允许参与以下的蛋白质的条件性组织特异性消融： 线粒体融合和分裂以及介导细胞凋亡以响应氧化细胞应激。

During the past funding cycle, the establishment of the services provided by the Cell Biology Core has fostered a large variety of research related to the Core Objectives. Cell Biological Studies related to the DRTC have expanded within the research community as evidenced by publications as well as grant applications from investigatoVs who traditionally do not work in the area of diabetes mellitus and metabolism. The adipose tissue bank and adipose biology expertise and service of the DRTC was transferred to operate under the auspices of the newly funded Mid-Atlantic nutrition and obesity research center (NORC) at the University of Maryland. This ensured a strengthening and continuity of adipose tissue related services to the DRTC community while freeing resources, which were used for innovation and expansion of services through the Cell Biology Core of the Baltimore DRTC. The Cell Biology Core started a local repository of transgenic mouse models of tissue/cell specific transgenic CRE -deleter ordoxycylcine regulatable mice. These well-characterized mice, in the C57BI/6 background, have become a valuable resource for the research community. Responding to investigators' needs as well as to pertinent questions related to metabolism and diabetes mellitus, the Cell Biology has expanded its services and expertise in the area of cellular bioenergetics and mitochondrial biology. This new addition will provide expertise and services to image and quantify mitochondrial content and mitochondrial shape and constellation, to assess mitochondrial dynamics (fusion and division), provide expertise in quantification of mitochondrial gene and protein expression (e.g. uncoupling proteins, complexes of the electron transfer chain) and cellular mitochondrial DNA content. In addition DRC users will gain access to unique genetic mouse models (e.g. floxed Drp1, floxeci Opal mice), which allow conditional tissue specific ablation of proteins involved in mitochondrial fusion and division and in mediating apoptosis in response to oxidative cellular stress.