Human Endometrial Tissue and DNA Bank

项目总结（见说明）： 我们在加州大学弗朗西斯科分校的NIH U 54 SCCPIR中心的这次更新提出了一种跨学科的团队科学方法，以阐明早期发育中种子事件的潜在机制，因为它们与人类不育的起源和生物学后果有关。我们的调查人员 重点关注从卵母细胞能力到胚胎和胎盘发育到植入的发育连续体，并促进生殖研究、先进技术、信息学数据分析、指导和社区参与方面的卓越和创新的共同愿景。我们结合联合收割机强大的实验技术，系统方法和计算分析，以解决关键问题，在 干细胞生物学、表观基因组学和临床生殖医学的交叉。为了实现这些目标，我们提出了四个高度互动的项目，由既定的调查员和一个试点项目，与一个新的调查员，这是由三个核心的支持。项目一（马尔科·孔蒂）侧重于机制 控制卵母细胞发育能力和早期胚胎发育（苏珊费舍尔）将研究滋养层分化的早期阶段的分子分析;项目三（罗伯特Blelloch）专注于滋养层分化的转录后调控;项目四（琳达朱迪斯）将研究胚胎植入的人类子宫内膜的发育;和试点项目（Nam Tran）建议研究子宫内膜异位症发病机制和病理生理学的分子机制，子宫内膜异位症是一种与不孕症和妊娠结局不良相关的常见疾病。（琳达朱迪斯），核心B为计算生物学研究（宋俊），核心C为教育和社区推广（辛西娅梅隆）。我们的长期 目标是从根本上了解正常和异常早期发育的机制，并制定新的战略来诊断，预防和治疗不孕症;优化生育和怀孕结果，并尽量减少不良后果;教育有抱负的学生，公众，卫生保健专业人员和患者关于人类发育和异常发育相关的疾病;并激励 在生殖健康和研究这一重要领域的职业选择和培养职业发展。 这些目标与国家排雷中心的使命是一致的。

PROJECT SUMMARY (See Instructions): This renewal of our NIH U54 SCCPIR Center at the University of California, San Francisco proposes a transdisciplinary, team science approach to elucidate mechanisms underlying seminal events in early development as they relate to the origins and biological consequences of human infertility. Our investigators focus on the developmental continuum from oocyte competence to embryo and placental development to implantation and promote a shared vision of excellence and innovation in reproductive research, advanced technologies, informatics data analyses, mentoring, and community engagement. We combine powerful experimental techniques, system approaches and computational analyses to address critical questions at the intersection of stem cell biology, epigenomics, and clinical reproductive medicine. To accomplish these goals, we have proposed four highly interactive projects by established investigators and one pilot project with a new investigator, which are supported by three cores. Project I (Marco Conti) focuses on mechanisms controlling oocyte developmental competence and early embryo development; Project II (Susan Fisher) will investigate a molecular analysis of the early stages of trophoblast differentiation; Project III (Robert Blelloch) focuses on post-transcriptional regulation of trophoblast differentiation; Project IV (Linda Giudice) will investigate development of human endometrium for embryonic implantation; and the Pilot Project (Nam Tran) proposes to investigate molecular mechanisms underlying the pathogenesis and pathophysiology of endometriosis, a common disorder in women associated with infertility and poor pregnancy outcomes These projects are supported by Core A for administration (Linda Giudice), Core B for computational biology research (Jun Song), and Core C for education and community outreach (Synthia Mellon). Our long-term goals are to understand mechanisms underlying normal and abnormal early development fundamentally and for developing novel strategies to diagnose, prevent and treat infertility; optimize fertility and pregnancy outcomes and minimize adverse outcomes; educate aspiring students, the public, health care professionals and patients about human development and disorders associated with abnormal development; and inspire career choice and nurture career development in this important area of reproductive health and research. These goals are consistent with the mission of the NICHD.