FUNCTIONAL GENOMICS

功能基因组学共享资源(FG)使癌症中心成员能够进行基因 以快速有效的方式在细胞中筛选。为此，FG建立了一个标准的工作流程， 从可行性评估、转基因优化、分析开发、中试筛选、全面展开开始 筛选、数据分析和确认已确定的目标。FG的最初关注点是siRNA 筛查，到目前为止，FG已经开展了19个项目，解决从基础癌症到基础癌症的各种问题 生物学对具有治疗潜力的化合物的活性分析。单独排列的siRNA文库 在核心包括一个大型的“人类可用药”集合，有4个独立的siRNA到7200个基因，一个类似的 小鼠集合，以及各种针对激酶、蛋白水解酶、GPCRs和a的聚焦siRNA文库 Burnham设计的1,100个基因靶“泛素”组后者支持强烈的泛素兴趣 信号转导计划中的小组。鉴于对功能基因组学日益增长的需求和作用 经过两年的发展，取得了较好的效果。功能基因组学现在被提议作为新的共享 资源。在下一个资助期内，FG计划增强其服务剧目，以包括增益功能 通过cDNA超表达、microRNA模拟和功能抑制物筛选 能力。该设施还将通过化学修饰的siRNA文库来增加基因覆盖率 整个人类基因组，并实施慢病毒介导的筛查方法，以能够解决 与癌症相关的细胞，难以转化，无法进行功能基因组学研究 目前的技术。功能基因组学共享资源利用卓越资源 可在化学基因组学中心(其中化学图书馆筛选共享资源是 位于)，包括液体处理、自动化、多模版阅读器和高含量筛选 设备和专业知识。在过去的一年。塔里克·拉纳博士加入了伯纳姆，他是 FG核心的主管。作为RNA干扰(RNAi)机制和RNA领域的知名领导者 生物学。拉纳博士的专业知识将为这一新的共享资源提供关键指导。 总体而言，第一年需要99 609美元的CCSG支助，占估计总数的20.3% 功能基因组学共享资源的年度运营预算。

The Functional Genomics Shared Resource (FG) enables Cancer Center members to perform genetic screens in cells in a rapid and effective manner. To this end, FG has established a standard workflow that starts with a feasibility assessment, transfection optimization, assay development, pilot screens, full-scale screening, data analysis, and verification of targets identified. The initial focus of FG has been siRNA screening, and to date FG has undertaken 19 projects addressing questions ranging from basic cancer biology to activity profiling of compounds with therapeutic potential. The individually arrayed siRNA Libraries in the core include a large "human druggable" collection, with 4 individual siRNAs to 7,200 genes, a similar mouse collection, and a variety of focused siRNA libraries targeting kinases, protease, GPCRs, and a Burnham-designed 1,100 gene target "Ubiquitinome" set The latter supports the strong ubiquitin interest group in the Signal Transduction Program. Given the increasing demand and role for functional genomics approaches, after two years of development. Functional Genomics is now proposed as new Shared Resource. During the next funding period, FG plans to enhance its services repertoire to include gain-offunction screening via cDNA over-expression as well as microRNA mimic and functional inhibitor screening capabilities. The facility will also increase gene coverage with a chemically modified siRNA library against the entire human genome, and implement lentiviral-mediated screening methods to be able to address cancer-relevant cells that are difficult to transfect and are out of reach for functional genomics studies using present technology. The Functional Genomics Shared Resource leverages the remarkable resources available in the Center for Chemical Genomics (in which the Chemical Library Screening Shared Resource is located), including liquid handling, automation, multimode plate readers, and high content screening equipment and expertise. In the past year. Dr. Tariq Rana joined Burnham and he serves as the Scientific Director of the FG core. As a renowned leader in the mechanisms of RNA interference (RNAi) and in RNA biology. Dr. Rana's expertise will provide critical guidance for this new Shared Resource. Overall, $99,609 in CCSG support is requested in the first year, representing 20.3% of the total estimated annual operating budget for the Functional Genomics Shared Resource.