Cell Biology and Histology

细胞生物学和组织学核心(CBHC)是这个PPG的关键资源，将被所有人使用 项目。CBHC将为项目提供以下服务： 1.心脏祖细胞的制备：心脏祖细胞的分离、鉴定和保存 野生型和转基因小鼠， 2.获得稳定表达CPC的慢病毒细胞系， 3.免疫组织病理学：为所有项目准备组织切片，评估组织病理学 心肌的大体和微观结构，心脏切片中细胞的荧光显微镜成像， 血管系统的分析。 CBHC将设在加州大学圣地亚哥分校。CBHC将容纳和维护 设备，并为项目提供训练有素的工作人员。它将为这四个项目提供访问 广泛的检测和分析，包括CPC的分离、分类和表征，CPC的分析 活体获得的心脏标本的功能和组织病理学。CBHC服务还将包括隔离、 项目所需的转基因小鼠CPC的维护特性。CBHC还将 扩展和鉴定萨斯曼博士分离的人类CPC，以供其他项目使用。CBHC将 确保所有四个项目都将使用以相同方式隔离、扩展和排序的CP。这 一致性对于整合和比较两国之间的结果极其重要 项目。CBHC还将协助所有项目准备组织切片和 组织病理学评估大体和微观心肌结构，包括纤维化程度， 腔大小和心肌细胞横截面积的定量。它还将分析心脏切片，以 TUNEL和抗脱落caspase-3免疫荧光染色检测到凋亡细胞； 通过以下方式提供心肌细胞结构和信号分子定位的微观评估 小鼠心脏切片的免疫荧光。最后，CBHC将对心脏切片进行染色以检测c-kit CPC阳性加上分化和增殖的标记物，并准备冠脉管型。统一 心脏组织切片的制备和分析也是保持一致性和 四个项目之间的可比性结果。 相关性(见说明)； 心肌细胞在组织缺氧时死亡，如心肌梗死(心脏 攻击)。在这里，我们分离出我们认为可以取代这些死亡细胞的祖细胞，并确定如何 以使他们能够更好地治愈心脏。我们还检查了这些细胞在心脏内的反应 对急性心肌梗死后心脏损害的影响。

The Cell Biology and Histology Core (CBHC) is a critical resource to this PPG and will be used by all Projects. The CBHC will provide the projects with the following services: 1. preparation of cardiac progenitor cells (CPCs): isolation, characterization, and storage of CPCs from wild type and genetically modified mice, 2. preparation of stable lentiviral expressing lines of CPCs, 3. immunohistopathology: Preparation of histological sections for all projects, histopathology to assess gross and microscopic myocardial structure, fluorescent microscopic imaging of CPCs in heart section, analysis of vasculature. The CBHC will be located at the University of California, San Diego. The CBHC will house and maintain the equipment and provide fully trained staff for the Projects. It will provide the four projects with access to a wide variety of assays and analyses, including CPC isolation, sorting and characterization, assays of CPC function, and histopathology of heart samples obtained in vivo. CBHC services will also include isolation, maintenance characterization of CPCs from genetically engineered mice for the projects. CBHC will also expand and characterize the human CPCs isolated by Dr. Sussman for use by other projects. CBHC will ensure that all four projects will use CPCs that have been isolated, expanded and sorted the same way. This consistency is extremely important for the integration and comparison of results between the projects. The CBHC will also assist all the Projects with preparation of histological sections and histopathology to assess gross and microscopic myocardial structure, including the extent of fibrosis, quantification of chamber size and myocyte cross sectional area. It will also analyze heart sections for presence of apoptotic cells by TUNEL and anti-deaved caspase-3 immunofluorescence staining, and provide microscopic assessment of cardiac cell structure and signaling molecule localization via immunofluorescence of mouse heart sections. Finally, the CBHC will stain heart sections to detect c-kit positive CPCs plus markers of differentiation and proliferation, and prepare coronary vascular casts. Uniform preparation and analysis of histological sections from hearts are also important for consistent and comparable results between the four projects. RELEVANCE (See instructions); Heart muscle cells die when the tissue lacks oxygen, as occurs with a myocardial infarction (Ml; heart attack). Here we isolate progenitor cells that we believe could replace these dying cells and determine how to make them better able to heal the heart. We also examine the response of these cells within the heart and their effects on cardiac damage following Ml.