Protein Chemistry Laboratory Core

蛋白质化学实验室核心（PCLC）将作为主要的蛋白质制备和分析 项目的资源。实验室将负责蛋白质制备、表征 和鉴定，其中将包括项目驱动的蛋白质组学。PCLC配备有肽 合成仪、冻干机、各种快速蛋白质液相色谱仪（FPLC）、高效液相色谱仪 高效液相色谱（HPLC）和超高效液相色谱（UPLC）系统涵盖微观和宏观尺度。最 重要的是，该实验室有一个“适合蛋白质组学”的质谱（MS）系统， 电离模式，将使亚皮摩尔分析的蛋白质，肽，和翻译后 在生物信息学的支持下进行PTM修饰。所有蛋白质和肽类产品将接受 通过质谱/蛋白质组学分析进行严格表征。表面等离子体共振（Biacore） 也可进行适于与现场质谱仪耦合的分析。主 提供的服务将是提供与蛋白质有关的工具和试剂，如纯化的补体 系统蛋白质、抗体、合成肽和肽样合成拮抗剂，其中断各种 和特异性补体系统蛋白质-蛋白质相互作用。此外，PCLC将执行基于MS的 这些试剂的分析沿着全面的样品分析和高通量筛选， PTM与补体/炎症过程相关。项目的所有成员将由 PCLC。

The Protein Chemistry Laboratory Core (PCLC) will serve as the major protein preparation and analysis resource for the Program Project. The laboratory will be responsible for protein preparation, characterization and identification, which will include project-driven proteomics. The PCLC is equipped with a peptide synthesizer, a lyophilizer, and various fast protein liquid chromatography (FPLC), high-performance LC (HPLC) and ultra-performance LC (UPLC) systems encompassing both micro and macro scales. Most importantly, the laboratory has a "proteomics-appropriate" mass spectrometry (MS) system equipped with ionization modes that will enable sub-picomole analysis of proteins, peptides, and post-translational modifications (PTM) with bio-informatics support. All protein and peptide products will be subjected to stringent characterization by mass spectrometric/proteomic analysis. Surface plasmon resonance (Biacore) analysis is also available that is adaptable to coupling with the on-site mass spectrometers. The primary services provided will be the provision of protein-related tools and reagents such as purified complement system proteins, antibodies, synthetic peptides, and peptide-like synthetic antagonists that interrupt various and specific complement system protein-protein interactions. In addition, the PCLC will perform MS-based analyses of these reagents along with comprehensive sample analysis and high-throughput screening of PTM related to complement/inflammatory processes. All members of the Program Project will be served by the PCLC.