CV7000

项目摘要/摘要 这项提案要求资金购买尖端的Wako CV7000高内容Conocus 斯坦福大学由美国国立卫生研究院资助的11名首席研究人员将共享成像系统 大学医学院。新系统有两个目的：它取代了租赁的 大量使用的自动荧光成像系统ImageXpress Micro XL和它 为斯坦福校园增加了一项关键的新功能，即进行自动共焦成像。这个 托拜厄斯·迈耶，詹姆斯·费雷尔，沈康，托马斯·旺德利斯，史蒂文·阿坦迪， Marius Wernig，Karlene Cimprich，Thomas Querterous，Michael Lin，Rajat Rohatgi，和 马修·斯科特位于很近的地方，他们对基于显微镜的研究有共同的兴趣 对细胞系统进行动态分析，以揭示和了解细胞周期、细胞 分化、细胞损伤、细胞迁移等细胞动态过程。中心是 我们实验室的研究是使用长期、活细胞、荧光显微镜来 扰乱、监测和量化细胞过程中的动态变化。具体来说，我们使用 需要自动跟踪细胞、监控细胞形状和细胞变化的方法 细胞器分布和染色质重塑的极化以及局部的变化 发信号。作为我们项目的一部分，我们还需要可视化和量化诱导表达和 调节和标记蛋白的降解以及信号蛋白的运动 以及往返于细胞核、质膜和其他细胞室的生物传感器。 去年，当制造商停止为我们的两个频繁使用的12- 已有一年历史的自动ImageXpress成像系统，我们能够获得一次性租赁 较新型号的ImageXpress Micro XL将于2013年8月推出。这个借贷系统的使用率很高。 每天24小时，因为我们的许多实验都需要1-3天的连续多井成像。 Wako CV7000的先进高通量活细胞成像功能将取代 这款借出的ImageXpress Micro XL，加上关键的共焦功能，还 显著增强NIH支持的实验室研究，通过提供共享 资源，以满足我们日益增长的显微镜需求。

PROJECT SUMMARY/ABSTRACT This proposal requests funds to purchase a cutting edge Wako CV7000 High-Content Confocal Imaging system to be shared by eleven NIH-funded Principal Investigators at Stanford University School of Medicine. The new system has two purposes: it replaces a leased automated epifluorescence imaging system ImageXpress micro XL that is heavily used and it adds a critical new capability to the Stanford campus to do automated confocal imaging. The laboratories of Tobias Meyer, James Ferrell, Kang Shen, Thomas Wandless, Steven Artandi, Marius Wernig, Karlene Cimprich, Thomas Quertermous, Michael Lin, Rajat Rohatgi, and Matthew Scott are located in close proximity and share a common interest in microscopy-based dynamic analysis of cellular systems to uncover and understand the cell cycle, cell differentiation, cell damage, cell migration and other dynamic cellular processes. Central to the research in our laboratories is the use of long-term, live-cell, fluorescence microscopy to perturb, monitor and quantify dynamic changes in cellular processes. Specifically, we use methods that require automated tracking of cells, monitoring of changes in cell shape and cell polarization, of organelle distribution and of chromatin remodeling and of changes in local signaling. As part of our projects, we also need to visualize and quantify induced expression and degradation of regulatory and marker proteins as well as the movement of signaling proteins and biosensors to and from the nucleus, plasma membrane and other cellular compartments. Last year, when the manufacturer discontinued all service support for our two heavily-used, 12- year old automated ImageXpress imaging systems, we were able to obtain a one-time lease on a newer-model ImageXpress Micro XL until August 2013. This loaner system is heavily used every day 24/7 since many of our experiments take 1-3 days of continuous multi-well imaging. The advanced high-throughput, live-cell imaging capabilities of the Wako CV7000 will replace this loaned ImageXpress Micro XL and, with the critical addition of confocal capabilities, also significantly enhance the NIH-supported research in our laboratories by providing a shared resource to address our increasing microscopy needs.