Genomics-Related Shared Resource Group

项目总结(见说明)： 生物检疫登记和处理共享资源(BAP)的总体目标是提供一个中央设施，以便以电子方式登记和处理拟供癌症中心成员研究的生物检疫登记。共享资源的具体目标是：1)将生物标本电子加入中央研究标本数据库，2)根据预计的最终用途对标本进行适当的处理，3)提供核酸提取服务，4)在安全和可访问的设施中提供经过处理的生物标本的长期储存，5)向癌症中心的研究人员提供咨询服务和特定的专业知识。通过这一资源获得的生物标本包括固体组织以及全血和其他体液。血液样本被处理用于核酸提取、单个核细胞的活的可控率冷冻和/或DNA、血浆、血清或黄褐色大衣的冷冻储存。DNA样本可以从机器人控制的冰柜中提取，等量放入试管或96孔板中进行下游分析，然后返回到存储中，而不需要研究人员付出多少努力。新鲜的固体组织被解剖并在液氮或冷冻介质中冷冻。BAP资源最有价值的贡献之一是标本注释，在标本加入时输入研究实验室信息系统(RLIMS)，随后用于及时和适当地检索存档标本。在目前的资助期间，BAP已经扩大到包括亚利桑那州梅奥诊所(MCA)和佛罗里达州梅奥诊所(MCF)的BAP实验室，成为一个三站点共享资源。2012年，罗切斯特BAP共享资源为梅奥诊所的300多名研究人员处理了100,000多条管子，并从23,000多个生物标本中提取了DNA，其中34%是MCCC成员。AZ-BAP处理了来自400多个IRB方案的近20,000个样本，代表86名梅奥诊所研究人员，其中2.1%是MCCC成员。FL-BAP为超过25名梅奥诊所的研究人员处理了10,000多份样本，其中75%是MCCC成员。

PROJECT SUMMARY (See instructions): The overall Objective of Biospecimens Accessioning and Processing Shared Resource (BAP) is to provide a central facility to electronically accession and process biospecimens intended for research by Cancer Center members. The Specific Aims of the Shared Resource are to: 1) provide electronic accessioning of biospecimens into a central Research Specimen Database, 2) to process the specimens appropriately according to their projected end use, 3) to provide nucleic acid extraction services, 4) to provide long term storage of processed Biospecimens in a secure and accessible facility, 5) to provide consultative services and specific expertise to Cancer Center investigators. Biospecimens accessioned through this resource include solid tissues as well as whole blood and other body fluids. Blood specimens are processed for nucleic acid extraction, viable controlled rate freezing of mononuclear cells, and/or frozen storage of DNA, plasma, serum or buffy coats. DNA samples can be retrieved from a robotically controlled freezer, aliquoted into tubes or 96 well plates for downstream analyses, and returned to storage with little effort required from the investigator. Fresh solid tissues are dissected and frozen in liquid nitrogen or freezing medium. One of the most valuable contributions of the BAP resource is the specimen annotation that is entered into the Research Laboratory Information System (RLIMS) at the time of specimen accessioning and subsequently utilized for timely and appropriate retrieval of archived specimens. During the current funding period, BAP has expanded to include the Mayo Clinic Arizona (MCA) and Mayo Clinic Florida (MCF) BAP labs to become a three-site Shared Resource. In 2012, Rochester BAP Shared Resource processed over 100,000 blood tubes and extracted DNA from over 23,000 biospecimens for over 300 investigators at the Mayo Clinic, 34% of which are MCCC members. AZ-BAP processed almost 20,000 samples from over 400 IRB protocols representing 86 Mayo Clinic investigators, 2 1% of which are MCCC members. FL-BAP processed over 10,000 samples for over 25 Mayo Clinic investigators, of which 75% are MCCC members.