Acquisition of a Cryoprobe for a 600 MHz NMR Spectrometer

描述(由申请者提供)：为支持纽约大学(NYU)的化学生物学和生物医学研究工作，包括化学系和医学院，申请资金为600 MHz核磁共振光谱仪购买低温探头。这些设备将被安置在化学系的生命科学共享仪器设施中。该设施是在NIH/NCRR以研究设施改善补助金的形式支持下建造的。拟议的低温探测器将支持9名NIH资助的用户，并将提供纽约大学目前无法获得的技术(即，校园内没有低温探测器)。低温探头将包括高灵敏度的1H和13C检测(增加3-4倍)，能够执行使用最先进的溶液核磁共振方法表征生物大分子结构动力学所需的所有三重共振实验。该设备还将允许样本量有限的研究人员进行敏感的结构研究。纽约大学在纽约结构生物学中心拥有部分成员资格，不足以满足纽约大学NIH资助的研究人员的核磁共振需求。因此，在校园里拥有一个低温探测器将有助于更多地获得这项技术，这将是揭示对人类健康和疾病至关重要的分子相互作用的关键。

DESCRIPTION (provided by applicant): Funds are requested to acquire a cryogenic probe for a 600 MHz NMR spectrometer to support chemical biology and biomedical research efforts across New York University (NYU), including the Department of Chemistry and the School of Medicine. The equipment will be housed in the Shared Instrumentation Facility for Life Science in the Department of Chemistry. This facility was built with support from the NIH/NCRR in the form of a Research Facilities Improvement Grant. The proposed cryogenic probe will support nine NIH funded users and will provide technology currently not available at NYU (i.e., no cryoprobe on campus). The cryogenic probe will include highly sensitive 1H and 13C detection (increase of 3-4-fold) with the ability to perform all triple resonance experiments needed to characterize the structural dynamics of biomacromolecules using state-of- the-art solution NMR methodology. This equipment will also allow researchers limited in sample mass to carry out sensitive structural studies. NYU has a partial membership at the New York Structural Biology Center that is insufficient to meet the NMR needs of the NIH-funded researchers at NYU. Therefore, having a cryogenic probe on campus will facilitate increased access to this technology that will be essential to revealing molecular interactions important in human health and disease.