Fluorescence Cytometry (FC)

项目摘要(见说明)：荧光细胞仪(FC)核心设备的任务是以经济高效的方式为研究人员提供最先进的技术，以帮助了解环境健康挑战中涉及的细胞过程。了解这一水平的过程使研究人员能够制定和测试关于环境因素如何影响健康的理论。核心是使用荧光标记和/或克隆抗体来研究细胞过程的仪器的所在地。BD FACSAria？是最先进的流式细胞仪和高速细胞分选机。三个激光器、十个滤光片组的配置允许同时分析多个分子信号的识别。此外，该仪器具有对感兴趣的亚群进行精确分类的能力，以供进一步研究。BD FACSCalibur是一种1激光器、3检测器的流式细胞仪，可提供5个参数的基本细胞分析。CompuCyte ICYS？激光扫描细胞仪(LSC)提供与流式细胞术类似的分子信号检测，不同之处在于样品固定在显微镜载玻片或细胞培养板上，而不是在液体悬浮液中。这允许分析更多类型的组织，并允许组织和单个细胞的可视化。Miltenyi Biotec AutoMacs？细胞分选机通过磁珠分离纯化亚群，以供进一步研究。Luminex？100？分析可寻址激光珠阵列，用于捕获和检测小样本中的多个分析物。蔡司荧光显微镜和成像系统用于可视化和捕获带有荧光标记的细胞的图像。除了提供可靠、维护良好的仪器设备外，核心还提供实验设计和分析方面的科学专业知识。仪器、试剂和方案技术的复杂性需要透彻的了解和培训。有一名核心主任和工作人员科学家监督项目，确保使用公认的方法取得可靠和可重复的结果。现有仪器和专业知识的结合使CEHS的研究人员以及蒙大拿大学的其他研究人员在出版和获得外部资金方面处于竞争优势。

PROJECT SUMMARY (See instructions): The mission of the Fluorescence Cytometry (FC) Core Facility is to provide investigators with state-of-the art technology in a cost-effective manner to aid in understanding cellular processes involved in environmental health challenges. Understanding the processes at this level enables researchers to formulate and test theories as to how environmental factors affect health. The Core is home to instruments that use fluorescent markers and/or monoclonal antibodies to investigate cellular processes. The BD FACSAria¿ is a state-of-the art flow cytometer and high-speed cell sorter. The three-laser, ten filter-set configuration allows the identification of multiple molecular signals to be analyzed simultaneously. Further, the instrument has capabilities of precise sorting of subpopulations of interest for further investigation. The BD FACSCalibur¿ is a 1-laser, 3-detector flow cytometer that provides basic cell analysis in 5 parameters. The CompuCyte iCys¿ Laser Scanning Cytometer (LSC) offers similar detection of molecular signals as in flow Cytometry, the difference being that the sample is fixed on a microscope slide or cell culture plates, rather than in a fluid suspension. This allows more types of tissues to be analyzed and permits visualization of tissues and individual cells. The Miltenyi Biotec autoMacs¿ cell sorter purifies subpopulations by magnetic bead separation for further investigation. The Luminex¿ 100¿ analyzes addressable laser bead arrays for capture and detection of multiple analytes in small samples. The Zeiss¿ Fluorescence Microscope and Imaging System is used to visualize and capture images of fluorescently tagged cells. In addition to providing reliable, well-maintained instrumentation, the Core offers scientific expertise in experiment design and analysis. The complexity of the technology of the instruments, reagents and protocols require a thorough understanding and training. Having a Core Director and Staff Scientist available to oversee projects ensures reliable and reproducible results using accepted methodologies. The combination of available instrumentation and expertise puts the researchers at CEHS as well as other investigators at the University of Montana at a competitive advantage in publishing and obtaining extramural funding.