Nanoparticle-based SERS assay for Dengue virus detection in blood

描述：登革热病毒（DENV）是一种蚊子传播的RNA病毒，可引起人类出血热、休克，严重时甚至死亡。DENV也被记录为一种输血传播病毒。AABB输血传播疾病委员会最近将DENV确定为美国具有输血传播潜在风险的高优先级新发感染性病原体。目前，没有针对登革热病毒的血液筛查措施。在大多数临床和研究实验室中，DENV的检测方法是核酸扩增法和酶联免疫吸附法（elisa）。核酸扩增成本高，elisa耗时长。因此，开发一种廉价、快速和敏感的检测DENV的方法对于降低输血介导感染的风险和改善及时监测至关重要。在这种需求的驱动下，在这个项目中，我们将开发一种基于金纳米粒子的、快速、敏感和经济高效的无标签表面增强拉曼光谱（SERS）检测方法，用于选择性检测血液中的DENV。我们预计该方法将使试剂成本降低到核酸扩增法的1/10，检出限可低于10个病毒/ml。此外，SERS分析可以发展成为一种自动筛选分析，适用于血液筛查设施。

DESCRIPTION: Dengue virus (DENV), a mosquito-transmitted RNA virus, can cause human hemorrhagic fever, shock and even death, in severe cases. DENV has also been documented as a blood transfusion-transmitted virus. AABB's Transfusion-Transmitted Diseases Committee has recently identified DENV as a high-priority emerging infectious agent with potential risk of transfusion transmission in the United States. Currently, there is no blood screening measure conducted for DENV. Detection methods for DENV in most clinical and research laboratories are nucleic acid amplification assays and enzyme-linked immunosorbent assays (ELISAs). The cost for nucleic acid amplification is high and ELISAs are time-consuming. Therefore, developing an inexpensive, fast, and sensitive test for detection of DENV is crucial to reduce the risk of transfusion-mediated infection and improve timely surveillance. Driven by this need, in this project, we will develop a gold nanoparticle-based, rapid, sensitive and cost-effective label-free Surface Enhanced Raman Spectroscopy (SERS) assay for selective DENV detection in blood. We expect this SERS assay will reduce the reagent cost to 1/10th the cost of nucleic acid amplification assay and detection limit can be below 10 viruses /ml. Furthermore, the SERS assay can be developed into an automated screening assay, which is suitable in blood screen facilities.