Hybrid Nanopore Platform for DNA Sequencing

描述(申请人提供)：拟议研究的目标是展示混合纳米孔结合自上而下的图案化和纳米制造与自下而上的生物组装。现有的纳米孔测序，特别是脂质双层中的生物纳米孔测序，以降低并行度和数据吞吐量为代价，实现了高速和读取长度。该平台的脆弱性也降低了它在商业化方面的实用性。该项目的主要目标是建立一种生物-固态混合结构，这种结构可以以稳健、自动化的方式高效地自组装。我们的初步研究已经确定了可能的生物通道载体，可以组装到~10 nm的固态纳米孔上。拟议的工作将侧重于展示纳米孔蛋白质的掺入、自限通道插入，以及利用混合平台展示生物纳米孔的活性。建议的平台保留了生物纳米孔系统的速度和读取长度特性，同时通过利用现有的半导体技术显著提高了并行化和系统稳定性。

DESCRIPTION (provided by applicant): The objective of the proposed research is to demonstrate hybrid nanopores combining top-down patterning and nanofabrication with bottom-up biological assembly. Existing nanopore sequencing, especially biological nanopores in lipid bilayers, achieve high speed and read length at the cost of decreased parallelization and data throughput. The fragility of the platform also makes it less practical in commercialization. The primary goal of this project is to establish a hybrid biological-solid state structure that can be self-assembled in a robust automated manner with high efficiency. Our preliminary research has identified plausible carriers of biological channels that can be assembled onto ~10nm solid-state nanopore. The proposed work will focus on demonstration of nanopore protein incorporation, self-limited channel insertion, and demonstration of biological nanopore activity with the hybrid platform. The proposed platform retains the speed and read length characteristics of biological nanopore systems while dramatically increasing the parallelization and system stability by leveraging established semiconductor technologies.