Time-keeping Mechanisms in Drosophila Embryonic Development

摘要 候选人目前是埃里克教授实验室的生命科学研究员 普林斯顿大学分子生物学系的Wieschaus博士说。候选人被授予 洛克菲勒大学的定量细胞生物学博士。Postdoctoral期间 奖学金，候选人正在过渡到发育生物学领域。候选人将申请 以前研究的定量和分析技术以及遗传学方面的进一步培训 和发育生物学，以研究胚胎发育过程中细胞行为的时间控制 发展NIH独立之路奖将为 在这个过渡时期的候选人。该奖项将使候选人获得新的技能， 遗传学和发育生物学以及建立新的研究方向。候选 将受益于机会采取研究生课程“多细胞生物遗传学”在 普林斯顿大学以及课程“真核基因表达”和“基因调控 冷泉港实验室和海洋生物实验室的“发展网络” 分别候选人将研究确保精确时间调节的分子机制 通过控制基因表达、信号传导和蛋白质降解来控制细胞分裂 胚胎发育在K99阶段的奖励，候选人将1）发展理论 分析信号系统的整合时间2）进行遗传筛选和分子生物学 鉴定Cdc25转录调节因子（细胞周期限速激活因子）的实验3） 确定Cdc25蛋白降解调节在母体到合子的重要性 转型，一个关键的发展转型。这些目标将通过结合遗传学， 胚胎学、分子生物学、定量活体成像和数学建模。在R00期间 在该奖项的第一阶段，候选人将通过确定分子机制来扩展研究 确保Cdc25以高时间精度转录和降解， 细胞周期控制之外的信号系统。候选人最终希望追求学术 从事研究和教学工作。

Abstract The candidate is currently a Life Science Research Fellow in the laboratory of Professor Eric Wieschaus in the Department of Molecular Biology at Princeton University. The candidate was awarded a PhD from The Rockefeller University for research in quantitative cell biology. During the Postdoctoral Fellowship, the candidate is transitioning to the field of developmental biology. The candidate will apply the quantitative and analytical techniques from previous research as well as further training in genetics and developmental biology to the study of the control of timing of cell behaviors during embryonic development. The NIH Pathway to Independence Award would provide necessary support to the candidate during this transition period. The award would allow the candidate to acquire new skills in genetics and developmental biology as well as to establish novel research directions. The candidate will benefit from the opportunity to take the graduate course 'Genetics of Multicellular Organisms' at Princeton University as well as the courses 'Eukaryotic Gene Expression' and 'Gene Regulatory Networks for Development' at Cold Spring Harbor Laboratory and at the Marine Biological Laboratory respectively. The candidate will study the molecular mechanisms ensuring precise temporal regulation of cell division through control of gene expression, signaling and protein degradation during Drosophila embryonic development. During the K99 phase of the award, the candidate will 1) Develop theoretical analysis of the integration time of signaling systems 2) Perform genetic screens and molecular biology experiments to identify regulators of Cdc25 transcription (rate-limiting activator of the cell cycle) 3) Determine the importance of regulation of Cdc25 protein degradation at the maternal-to-zygotic transition, a critical developmental transition. These aims will be accomplished by combining genetics, embryology, molecular biology, quantitative live imaging and mathematical modeling. During the R00 phase of the award, the candidate will extend the research by determining the molecular mechanisms ensuring that Cdc25 is transcribed and degraded with high temporal precision and by analyzing signaling systems beyond cell cycle control. The candidate ultimately desires to pursue an academic career in research and teaching.