CELLULAR IMAGING, SURGERY, AND TISSUE PROCESSING CORE FACILITY

项目摘要(参见说明)：核心B对于支持本PPG中使用的显微成像、定量分析和周围神经损伤模型至关重要。核心被所有三个项目广泛使用，并直接为这些项目的多个目标作出贡献。核心B的范围和资源已通过增加或更新新仪器(例如，由PPG行政补编资助的电子显微镜AMT数码相机)得到显著扩展，以满足PPG项目的需要。一个重要的补充是提供专门知识和技术帮助，以提供所有3个项目都使用的啮齿动物周围神经损伤的单一模型。这消除了手术技术和在特定肌肉或神经中微量注射示踪剂项目之间的变异性，并促进了项目之间数据的直接比较，以帮助解释。核心设施由一名经验丰富的调查员和研究管理人员领导，并由三名训练有素的合格研究助理协助。核心向PPG小组成员提供所有必要的培训，以获得授权使用核心仪器和设施；PPG成员可优先使用该设施。服务包括培训和指导仪器和分析技术的使用，协助设计和/或解释实验，按要求实际操作组织处理和成像，电子显微镜处理和成像，以及显微手术和示踪剂注射。重要的资源包括3台共焦显微镜(一台是具有电生理设置的多光子仪器)、电子显微镜套件、NeuroLucida系统、荧光解剖显微镜、低温恒温器、振动体和超微结构、冷冻替代系统、设备齐全的组织实验室、用于啮齿类动物的最先进的显微外科手术套装，以及四个用于图像处理和数据分析的离线工作站。数据被安全地存储，每个PPG PI都可以安全地访问PPG共享数据云(1.0 TB)，在那里他们可以访问自己和彼此的数据，并与Core中的数据采集仪器进行无缝通信。

PROJECT SUMMARY (See Instructions): Core B is essential to support the microscopic imaging, quantitative analysis, and peripheral nerve injury model used in this PPG. The Core is used extensively by all three projects and contributes directly to multiple goals of these projects. The scope and resources of Core B have been significantly expanded by addition or update of new instrumentation (e.g. an AMT digital camera for the electron microscope, funded by an Administrative Supplement to the PPG) to meet the needs of the PPG projects. A significant addition is the provision of expertise and technical help to provide a single model of peripheral nerve injury in rodents that is used by all 3 projects. This eliminates variability between projects in surgical technique and microinjection of tracers in specific muscles or nerves, and facilitates direct comparison of data between projects to aid in interpretation. The Core facility is directed by a highly experienced investigator and research administrator, assisted by three highly trained and qualified research associates. The Core provides all necessary training to members of the PPG groups for authorized access and use of Core instruments and facilities; PPG members have priority access to the facility. Services include training and guidance for use of instruments and analytical techniques, assistance with design and/or interpretation of experiments, hands-on performance of tissue processing and imaging as required, electron microscopy processing and imaging, and microsurgery and tracer injection. Significant resources include 3 confocal microscopes (one is a multiphoton instrument with electrophysiology setup), electron microscope suite, Neurolucida system, fluorescence dissecting microscope, cryostats, vibratomes and ultramicrotomes, cryosubstitution system, a fully equipped histology laboratory, access to a state-of-the-art suite for microsurgery in rodents, and four off-line workstations for image processing and data analysis. Data is securely stored, and each PPG PI has secure access to a PPG shared data Cloud (1.0 terabyte) where they can access their own and each other's data and communicate seamlessly with the data acquisition instruments in the Core.