Validation of LC-MS/MS analyses of animal tissue and feed matrices for toxicants

2013年Gaskill Vet-LIRN项目摘要/摘要 兽医实验室调查和反应网络(VET-LIRN)是由FDA开发的，目的是 通过调查涉及动物饲料的潜在不良事件，提高人类和动物健康的安全性 或动物药物污染或掺假。对诊断样本的检测增加了对 不是从传统食品检测实验室常规获得的调查。这样的调查需要 对器官和诊断样本(如尿液、粪便和血液)进行验证的检测方法， 不是典型的食物基质。 拟议工作的目标是扩大多实验室验证的分析方法的数量。 可由FDA Vet-LIRN合作实验室在疫情或动物饲料或 毒品污染。拟议的项目还将加强 网络实验室，鼓励在实际紧急情况相关测试期间进行无缝交互。 这项建议的具体目标是执行多个实验室的液体色谱-串联验证 动物体内抗凝血灭鼠剂的LC-MS/MS检测方法 组织、液体和饲料，以及动物组织和饲料中的真菌毒素。它们的分析物和矩阵 将在项目的最后一年根据需要扩展方法，以满足Vet-LIRN的具体需求。 该提案的另一个主要目的是为其他资助的VET-LIRN多 实验室方法验证项目。我们已经同意合作并为其他七名兽医提供支持- LIRN网络毒理学实验室。这项提案将有助于确保肯塔基大学 兽医诊断实验室拥有必要的仪器和技术人员支持，以充分 处理协作分析。 预期成果是完成上述方法的多实验室验证，并 向Vet-LIRN合作实验室和FDA Vet-LIRN传播方法。经济衰退带来的影响 拟议的工作将是通过增加有效测试的数量来改善人类和动物的健康 可供Vet-LIRN合作实验室在暴发或动物饲料或 药物污染或掺假。

2013 Gaskill Vet-LIRN Project Summary/Abstract The Veterinary Laboratory Investigation and Response Network (Vet-LIRN) was developed by the FDA to increase the safety of human and animal health by investigating potential adverse events involving animal feed or animal drug contamination or adulteration. The testing of diagnostic specimens adds insight into investigations not routinely obtained from traditional food testing laboratories. Such investigations require detection methods that are validated for organs and diagnostic samples such as urine, feces, and blood, which are not typical food matrices. The objective of the proposed work is to expand the number of multi-laboratory validated analytical methods that can be used by the FDA Vet-LIRN cooperative laboratories during outbreaks or events of animal feed or drug contaminations. The proposed project will also strengthen the collaborations and integration of the network laboratories to encourage seamless interactions during actual emergency related testing. The specific aim of this proposal is to perform multi-laboratory validations of liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for detection and quantitation of anticoagulant rodenticides in animal tissues, fluids and feeds, and mycotoxins in animal tissues and feeds. Analytes and matrices for these methods will be expanded in the last year of the project as needed to address specific Vet-LIRN needs. Another major aim of this proposal is to provide collaborative support for other funded Vet-LIRN multi- laboratory method validation projects. We have agreed to collaborate and provide support of seven other Vet- LIRN network laboratory toxicology sections. This proposal will help ensure that the University of Kentucky Veterinary Diagnostic Laboratory has the necessary instrumentation and technical staff support to adequately handle the collaborative analyses. The expected outcomes are to complete the multi-laboratory validation of the methods listed above and disseminate the methods to Vet-LIRN collaborating laboratories and to the FDA Vet-LIRN. The impact of the proposed work will be to improve human and animal health by increasing the number of validated testing methods available for collaborating Vet-LIRN laboratories to use during outbreaks or events of animal feed or drug contamination or adulterations.