Cell Imaging and Analysis Facility CORE

细胞成像和分析设施核心的使命是提供最先进的仪器和共聚焦显微镜，流式细胞术，细胞分选和组织病理学的专业知识。 理由 对于研究环境毒素影响组织、细胞和亚细胞器的机制的研究人员来说，成像是一种不可或缺的调查模式。最近的进展，如荧光探针，绿色荧光蛋白（GFP），共聚焦光学，双光子光学，光电探测器，提高了我们的能力，图像复杂的组织。它们使我们能够可视化单个分子并监测活细胞中的分子相互作用。成本和复杂性问题使得个体研究者获得最先进的成像技术是不切实际的。因此，需要一个有组织的专家组，在成像技术方面的专业知识，以获得仪器，掌握应用程序，并帮助个人研究人员应用该技术来回答特定的研究问题。 服务 我们支持EHSC调查员完成这些任务： 通过由经验丰富的兽医病理学家提供专家病理分析，确定暴露于环境毒素和其他治疗造成的损害。 通过提供仪器、分析软件、基础设施和技术援助，获取斑马鱼和其他模型的高分辨率图像。 通过使用流式细胞术、显微镜和质谱法开发先进的方法来量化信号转导途径的激活状态。 通过提供仪器和技术援助，从细胞培养物、血液和组织中分离特定的细胞群。

The mission of the Cell Imaging and Analysis Facility Core is to provide access to state-of-the-art instrumentation and expertise in confocal microscopy, flow cytometry, cell sorting, and histopathology. Rationale Imaging is an indispensable mode of inquiry for researchers studying the mechanisms by which environmental toxins affect tissues, cells, and subcellular organelles. Recent advances such as fluorescent probes, green fluorescent protein (GFP), confocal optics, two-photon optics, and photoelectronic detectors have improved our ability to image complex tissues. They have enabled us to visualize single molecules and to monitor molecular interactions in live cells. Cost and complexity issues make it impractical for individual investigators to obtain state-of-the-art imaging technologies. Therefore, the need exists for an organized group of specialists with expertise in imaging technologies to acquire the instrumentation, master the applications, and help individual investigators apply the technology to answer specific research questions. Services We support EHSC Investigators to accomplish these tasks: ¿ Determine the damage resulting from exposure to environmental toxins and other treatments by providing expert pathological analysis from an experienced Veterinary Pathologist. ¿ Acquire high-resolution images of zebrafish and other models by providing instrumentation, analysis software, infrastructure, and technical assistance. ¿ Quantify the activation state of signal transduction pathways by developing advanced methodology using flow cytometry, microscopy, and mass spectrometry. ¿ Isolate specific cell populations from cell culture, blood, and tissue by providing instrumentation and technical assistance.