Bioinformatics Core

核心B(生物信息学)的主要目标是为该计划提供计算工具和 处理、分析、整合和解释复杂的ChlP-Seq和基因表达所需的专业知识 分析从正常和恶性T细胞前体细胞获得的数据集。到目前为止， 这些领域是在特别的基础上提供的。创建此核心将为计划提供 专门的纵向信息学和生物统计学支持，这对该计划的整体 成功。该实验室由X.Shirley Liu博士领导，他是一位经验丰富、高效的计算专家 生物学家和生物统计学家，在癌细胞计算分析方面有创新的记录 表观遗传学和基因调控。其主要职责将包括分析获得的ChlP-Seq数据。 来自人和小鼠T-ALL细胞系，人和小鼠初级T-ALL，以及正常的人和 小鼠胸腺细胞亚群；从这些细胞获得的基因表达谱和/或RNAseq的分析； 并对这些ChlP-Seq和基因表达数据集进行了集成建模。 核心B在下一个项目期的具体目标如下： 目的1.处理、分析和整合来自正常和基因表达的ChlP-Seq和基因表达数据 恶性T细胞前体细胞 目的2.表征和量化与T细胞分化或扰动相关的表观遗传学变化 T-ALL细胞中转录因子的表达 目标3.就研究设计和计算/技术进步向项目PI提供建议 目标4.作为项目和生物医学研究的数据存储库和资源中心 社区作为一个整体

The major goals ofthe Core B (Bioinformatics) are to provide the Program with the computational tools and expertise needed to process, analyze, integrate and interpret complex ChlP-Seq and gene expression profiling datasets obtained from normal and malignant T cell progenitors. To date, programmatic needs in these areas have been provided on an ad hoc basis. Creating this Core will provide the Program with dedicated longitudinal informatics and biostatistical support, which is important for the Program's overall success. The facility is headed by Dr. X. Shirley Liu, an experienced, highly productive computational biologist and biostatistician with a track record of innovation in computational analysis of cancer cell epigenetics and gene regulation. Its principle responsibilities will include analysis of ChlP-Seq data obtained from human and murine T-ALL cell lines, human and murine pnmary T-ALLs, and nonnal human and murine thymocyte subsets; analysis of gene expression profiles and/or RNASeq obtained from these cells; and integrative modeling ofthese ChlP-Seq and gene expression datasets. The specific aims of Core B in the next project period are as follows: Aim 1. To process, analyze, and integrate ChlP-Seq and gene expression datasets from normal and malignant T cell progenitors Aim 2. To characterize and quantify epigenetic changes associated with T cell differentiation or perturbation of transcription factors in T-ALL cells Aim 3. To advise Program Pis on study design and computational/technological advances Aim 4. To serve as a data repository and resource center for the program and the biomedical research community as a whole