Gut Microbiome in Adults with Early Life Exposures to Environmental Chemicals

描述（由申请人提供）：微生物组的特征与发育和出生后暴露于环境化学物质以及与葡萄糖代谢异常标志物的可能关联将在bbbb500名年轻人中进行检查，这些年轻人是1986-1987年在法罗群岛建立的一个独特的出生队列的成员，法罗群岛是一个环境化学物质暴露范围异常广泛的社区。将邀请年龄为28岁的队列成员进行临床检查，并收集粪便样本。在出生时以及在7岁、14岁和22岁时的临床检查中获得了过去的化学品暴露、饮食、病史和其他协变量的数据，其中84%-90%的队列（N = 1,000）参与了这些数据。这些数据包括甲基汞、多氯联苯、DDE和全氟化合物的暴露情况。临床检查包括葡萄糖耐量试验。这些受试者的肠道微生物群的结构和功能将分两个阶段进行评估。每个样本中存在的微生物将通过16S rRNA基因测序确定，然后对选择的样本子集进行深度宏基因组和亚转录组测序，以最大限度地提供生物学信息。结合下游统计生物标志物的发现，这将允许确定与生命早期化学暴露和随后的成人葡萄糖耐量相关的微生物分支、基因、途径或转录本。

DESCRIPTION (provided by applicant): The characteristics of the microbiome in regard to developmental and postnatal exposure to environmental chemicals and the possible associations with markers of glucose metabolism abnormalities will be examined in >500 young adults, who are members of a unique birth cohort established in 1986-1987 at the Faroe Islands, a community with an unusually wide range of exposures to environmental chemicals. Cohort members will be invited at age 28 years for clinical examination, and a fecal sample will be obtained. Data on past chemical exposures, diet, medical history and other covariates were obtained at birth and at previous clinical examinations at ages 7, 14, and 22 years, in which 84%-90% of the cohort (N = 1,000) participated. The data include exposures to methylmercury, PCBs, DDE, and perfluorinated compounds. The clinical examination will include glucose tolerance test. The structure and function of the gut microbiome of these subjects will be assessed in a two-stage process. The microbes present in every sample will be determined by 16S rRNA gene sequencing, followed by deep metagenomic and metatranscriptomic sequencing on a subset of samples chosen to be maximally biologically informative. In combination with downstream statistical biomarker discovery, this will allow the determination of microbial clades, genes, pathways, or transcripts associated with early life chemical exposure and subsequent adult glucose tolerance.