MICROFLUIDICS AND SYNTHETIC BIOLOGY CORE

研究核心B-摘要 微流体和合成生物学核心将提供最先进的技术和专业知识 制造和使用生命科学中的高通量、单细胞数据采集设备。《核心》 将协助用户设计和制造定制的微流控器件，为用户提供 收集高质量的单细胞数据并与网络密切合作所需的微观资源 汇编和数学建模核心(Research Core C)，帮助用户分析他们的数据。《核心》 将通过四个具体目标完成这些任务。首先，核心目标是快速可靠地创建 通过利用最先进的制造设施为用户提供实验工具。它将寻求增加 通过开发和分发易于使用、可靠的微流控设备在生命科学中的应用 为用户的研究需求量身定做的微流体设备。第二，它将进行尖端 微流体学研究用于新生物和实验室技术的改进装置。《核心》 计划开发用于分离和培养环境中本地细菌的微流控设备 样本，以及能够在一个种群中选择有益性状的大规模进化装置 微生物。第三，它的目的是教育生命科学界的成员在开发、生产 以及微流控设备的使用。它将为中心成员提供许多学习的机会 微流控制造技术以及使用这些工具所需的实验技能。第四 最后，核心将开发和分发开源硬件和软件，以支持 微流体学研究。我们将效仿我们成功的拨号a Wave系统，这是完全 在一个免费访问的网页上进行了在线描述。有关组装硬件的详细说明，请参阅 为中心和其他机构的研究小组提供定制系统。 核心领导杰夫·哈蒂斯博士和实验室主管迈克尔·费里博士是该领域的领导者 合成生物学和微流控工具在生命科学中的应用。他们将一起继续他们的工作 协助所有对探索这些强大设备感兴趣的研究人员进行研究。

RESEARCH CORE B – SUMMARY The Microfluidics and Synthetic Biology Core will provide state-of-the-art technology and expertise for the manufacture and use of high throughput, single-cell data collection devices in the life sciences. The Core will assist users in designing and manufacturing custom microfluidic devices, provide users with the microscopic resources needed to gather high quality, single-cell data, and work closely with the Network Assembly and Mathematical Modeling Core (Research Core C) to help users analyze their data. The Core will accomplish these tasks through four Specific Aims. First, the core aims to rapidly and reliably create experimental tools for users by leveraging a state-of-the-art manufacturing facility. It will seek to increase the use of microfluidic devices in the life sciences by developing and distributing easy-to-use, reliable microfluidic devices that are custom made for research needs of users. Second, it will conduct cutting edge microfluidics research into improved devices for novel organisms and laboratory techniques. The Core plans on developing microfluidic devices for isolating and culturing native bacteria from environmental samples, as well as large-scale evolution devices capable of selecting beneficial traits in a population of microbes. Third, it aims to educate members of the life sciences community in the development, production and use of microfluidic devices. It will provide numerous opportunities for Center members to learn microfluidic manufacturing techniques and the necessary experimental skills for using these tools. Fourth and finally, the Core will develop and distribute open source hardware and software for supporting microfluidics research. We will model this effort after our successful dial-a-wave system, which is fully described online in a freely-accessible webpage. Detailed instructions for assembling hardware will be provided as well as made-to-order systems for research groups both in the Center and at other institutions. The Core leader, Dr. Jeff Hasty, and the lab's supervisor, Dr. Michael Ferry, are leaders in the field of synthetic biology and the use of microfluidics tools in the life sciences. Together they will continue their work assisting the research of all investigators interested in exploring these powerful devices.