Flow Cytometry

摘要-流式细胞术共享资源 共享资源负责人：Jordi Ochando，博士 Tisch癌症研究所（TCI）流式细胞术共享资源设施（FCSRF）为TCI成员提供 获得最先进的技术和专门知识，以成功开展科学研究 项目流式细胞术是一种强大的技术，使研究人员能够同时研究 每秒数千个细胞的多参数特性，以破译复杂的细胞和分子 健康和疾病的过程。FCSRF为常规应用提供服务，例如单细胞 分离快速分离活细胞进行更创新和复杂的生物学研究， 代谢组学和脂质组学分析。FCSRF由机构流式细胞仪共享 资源设施和两个TCI卫星站点。FCSRF配备4台分析仪、2台小鼠细胞分选仪、3台 生物安全2+级区域中的细胞分选仪，专用于分离未固定的人类样本和小鼠样本 用II类病原体感染的细胞，和1个质谱细胞计数器（CyTOF）。此外，还有两个卫星站， 配备4台分析仪。配备7名流式细胞仪和质谱仪专家，100%致力于此 SRF提供涵盖流式细胞术各个方面的操作、培训和咨询服务 包括细胞周期分析，从癌症患者中分离单细胞，以及鉴定30多种不同的 细胞表面/细胞内抗原在同一细胞上的检测。考虑到这一点，我们的使命是 提供有关流式/质谱细胞术和细胞分选的必要仪器和知识， Tisch癌症研究所的研究人员通过基础科学改善人类癌症的诊断和治疗， 转化和临床研究。

ABSTRACT – FLOW CYTOMETRY SHARED RESOURCE Shared Resource Leader: Jordi Ochando, PhD The Tisch Cancer Institute (TCI) Flow Cytometry Shared Resource Facility (FCSRF) provides TCI members access to state-of-the-art technology and expertise for the successful development of scientific research projects. Flow cytometry is a powerful technology that enables researchers to study simultaneous multiparametric characteristics of thousands of cells per second to decipher complex cellular and molecular processes in health and disease. The FCSRF offers services for routine applications such as single cell isolation to rapidly isolate live cells conduct more innovative and complex biological studies such as RNAseq, metabolomics, and lipidomics assays. The FCSRF is comprised of the institutional Flow Cytometry Shared Resource Facility and two TCI satellite sites. The FCSRF is equipped with 4 analyzers, 2 mouse cell sorters, 3 cell sorters contained in biosafety level 2+ areas dedicated to isolate non-fixed human samples and mouse cells infected with class II pathogens, and 1 mass cytometer (CyTOF). In addition, two satellite sites are equipped with 4 analyzers. Staffed with 7 flow and mass cytometry specialists committed at 100% to this facility, the SRF provides operational, training, and consultation services covering all aspects of flow cytometry including cell cycle analysis, isolation single cells from cancer patients, and identification of over 30 different cell surface/intracellular antigens on the same cell using mass cytometry. With this in mind, our mission is to provide the necessary instrumentation and knowledge regarding flow/mass cytometry and cell sorting so that Tisch Cancer Institute investigators improve human cancer diagnosis and treatment through basic sciences, translational, and clinical research.