Fluorescence Microscopy and Cell Imaging Shared Resource

荧光显微镜与细胞成像共享资源 摘要 荧光显微镜和细胞成像资源提供完整的荧光显微镜成像 服务。该癌症中心托管资源致力于支持创新、重要的癌症研究 促进学员发展。该资源为成员提供对经济高效的服务的访问， 精密的仪器，在细胞和组织的定量生物分子成像方面的卓越专业知识。 学院主任Angela Wandinger-Ness博士监督该资源，该资源由专家工作人员运营， 有38年的光学显微镜工作经验。资源检测集中在第二个 癌症研究设施和癌症中心管理的基于网络的预约系统(ILabs) 跟踪使用情况和账单。超分辨率(纳米尺度)生物分子成像的尖端技术， 用于四维粒子跟踪的高光谱成像和光片显微镜(正在开发中)是 通过谈判达成的协议提供。更常规的应用程序可以通过一系列 直立和倒置的蔡司、尼康和奥林巴斯显微镜。两个蔡司元共焦扫描头以及 奥林巴斯DSU旋转磁盘系统能够对固定细胞和活细胞进行共焦和多光子成像，并 纸巾。激光和扫描头配置允许在可见光范围内成像多达八种颜色、FRET、FRAP 和红外线激发。CRI Nuance光谱相机、Andor Ixon EMCCD和新的滨松闪光灯 4.0 sCMOS摄像头提供超高的速度和分辨率、低光敏感度和光谱图像 广域乐器收藏。用于膜事件可视化的TIRF能力，单粒子 定制微流体室中的跟踪和成像完善了共享的顶级功能 资源。根据成员的需要定期升级工具，并通过成功的 征集外部资金和联发办的支助，例如在上一年新购置的多光子仪器 资金周期。多个软件包允许图像去卷积、体视学和定量评估 所有收集的数据。不经常使用的用户可以请求专家工作人员提供全方位的成像服务。费用是 可与其他机构相媲美。资源人员通过结合以下方式促进癌症研究的卓越 最新的创新技术和仪器，并就最佳做法提供个性化建议 用于样品制备、探针选择和定量测量。关于资源的教育和传播 功能是多模式的：从基于Web的资源和教程到定制、有针对性的讲座和动手- 在研究生课程上。在上一个5年筹资期间，来自所有4个联合国排雷行动中心的48个癌症中心实验室 研究方案使用了该资源，共出版了89份出版物，其中12份目前尚待出版 PMCID。在本报告所述期间(2013年7月至2014年6月)，联东特派团成员占总数的67% 资源使用情况，并得到132项同行审查赠款的支持。

FLUORESCENCE MICROSCOPY & CELL IMAGING SHARED RESOURCE ABSTRACT The Fluorescence Microscopy and Cell Imaging Resource offers complete fluorescence microscopy imaging services. This Cancer Center managed resource is dedicated to enabling innovative, significant cancer research and promoting trainee development. The Resource provides member access to cost-effective services, sophisticated instrumentation, and salient expertise in quantitative, biomolecular imaging of cells and tissues. Faculty Director Angela Wandinger-Ness, PhD supervises the Resource, which is operated by expert staff with 38 years combined experience in light microscopy. Resource instrumentation is centrally located on the second floor of the Cancer Research Facility and a Cancer Center managed, web-based reservation system (iLabs) tracks usage and billing. Cutting-edge technologies for biomolecular imaging at super-resolution (nm scale), hyperspectral imaging for four-dimensional particle tracking, and light sheet microscopy (in development) are offered through negotiated agreements. More routine applications are readily accommodated by an array of upright and inverted Zeiss, Nikon and Olympus microscopes. Two Zeiss META confocal scanheads as well as an Olympus DSU spinning disk system enable confocal and multi-photon imaging of both fixed and live cells and tissues. Laser and scanhead configurations allow imaging of up to eight colors in the visible range, FRET, FRAP and infrared excitation. A CRi Nuance spectral camera, an Andor iXon EMCCD and a new Hamamatsu Flash 4.0 sCMOS camera offer exceptional high speed and resolution, low light sensitivity, and spectral image collection on wide-field instruments. TIRF capabilities for visualization of membrane events, single particle tracking and imaging in custom microfluidics chambers round out the top-flight capabilities of the Shared Resource. Instrumentation is regularly upgraded, in accordance with member needs, and through successful solicitation of extramural funding and UNMCC support, e.g. multi-photon instrument newly acquired in previous funding cycle. Multiple software packages allow image deconvolution, stereology, and quantitative evaluation of all collected data. Infrequent users may request full service imaging by expert staff members. Fees are comparable to other institutions. Resource personnel promote excellence in cancer research by incorporating the latest innovative technologies and instrumentation and by giving personalized advisement on best practices for sample preparation, probe selection and quantitative measures. Education and dissemination about resource capabilities is multi-modal: ranging from web-based resources and tours to custom, targeted lectures, and hands- on graduate courses. During the previous 5-yr funding period, 48 Cancer Center laboratories from all 4 UNMCC Research Programs used the Resource, resulting in a total of 89 publications, of which 12 are presently pending PMCIDs. In the reporting period (July 2013 – June 2014), UNMCC members were responsible for 67% of total Resource usage and were supported by 132 peer-reviewed grants.