Digital Microfluidics SAWN

 描述(由申请人提供)：本提案适用于DMF-SAWN(数字微流体-表面声波雾化)的生物医学应用，这是一种高度灵活的芯片实验室设备，可直接与质谱仪(MS)耦合。DMF避免了生产精细操作所必需的传统微阀网络所需的时间密集型劳动力 体液。无死体积的准备步骤很容易集成，不需要封闭的微通道，随着时间的推移，微通道可能会因碎片或表面粘连而堵塞。在DMF中，金属电极阵列被图案化在介质表面，然后用疏水层进行处理。与基于微通道的流体相比，DMF可以操作更大范围的体积(NL-mL)，并提供简化的多步骤样品处理，例如但不限于提取、纯化、重构和消化。由于DMF的机制依赖于介质衬底，这提供了使用最近的 开发了称为表面声波雾化(SAWN)的平面电离技术。与ESI相比，SAW具有以下优势：(1)像DMF一样，它是平面的，使得两者可以很容易地耦合，而无需毛细管基流体的专业知识；(2)由于消除了氧化还原化学产物，因此在ESI锯切中没有使用高直流电压会产生更高的信号/噪声；(3)液滴大小与应用频率成反比，从而允许根据需要定制液滴大小；以及(4)可以在不连续模式下操作，从而允许对纳升体积样品进行分析。这项提议的完成将导致MS离子源产品包含芯片上实验室。目标市场是质谱学，主要用于生物医学研究和临床诊断。

 DESCRIPTION (provided by applicant): This proposal is for biomedical applications of DMF-SAWN (Digital Microfluidics - Surface Acoustic Wave Nebulization), a highly flexible lab-on-a-chip device that can be directly coupled to mass spectrometry (MS). DMF circumvents the need for time intensive labor to produce traditional micro valve networks essential in fine manipulation of fluids. Dead volume-free preparatory steps are easily integrated without the need for enclosed micro channels, which over time can clog from debris or surface adhesion build- up. In DMF, arrays of metal electrodes are patterned on a dielectric surface and then treated with a hydrophobic layer. DMF can manipulate a greater range of volumes (nL-mL) than micro channel-based fluidics and offers streamlined multi-step sample processing such as, but not limited to extraction, purification, reconstitution, and digestion. Because the mechanism of DMF relies on a dielectric substrate, this offers a unique opportunity to couple to MS using a recently developed planar ionization technique called surface acoustic wave nebulization (SAWN). The advantages SAWN over ESI: (1) like DMF it is planar allowing the two to be readily coupled without expert knowledge of capillary based fluidics, (2) absence of the high DC voltage used in ESI SAWN produces higher signal / noise due to the elimination of redox chemistry products, (3) droplet size is inversely proportional to applied frequency allowing droplet size to be tailore to suit the need, and (4) it may be operated in a discontinuous mode allowing nanoliter-volume samples to be analyzed. Completion of this proposal will results in an MS ion source product incorporating a lab-on-a-chip. The target market is mass spectrometry, primarily for biomedical research and clinical diagnostics.