A new device to improve screening of corneal transplant tissue

詹姆斯·马鲁斯眼库内皮细胞计数仪 摘要 采购供移植用的供体角膜组织是一项重要的社会服务 资金严重不足的眼库组织不仅被收获，而且必须被筛选， 无数的问题，可能使组织不适合移植或构成健康风险， 组织受体。供体组织可能因形态学问题而被拒绝，包括先前的屈光不正 手术（LASIK）或低内皮细胞计数。经过手术的供体组织 留下上皮瓣，如LASIK，不能用于移植，因为瓣将分离 期间，低内皮细胞计数也是供体组织排斥的基础， 内皮细胞计数是实质性角膜水肿的前兆。 目前，眼库在组织被采集后对捐赠组织进行筛选， 作为整个地球仪或角膜-巩膜边缘。将组织送回眼库实验室， 进行各种测试，包括裂隙灯检查以确定组织功效， 角膜内皮显微镜检查以确定内皮细胞计数。 在先前资助的项目中，VOL开发了一种便携式眼部扫描仪设备，用于筛选组织， 在组织采集之前，在现场进行屈光手术。该装置不仅产生 确定屈光手术存在的明确指标，而且还降低了成本 如果组织被认为是不可用的，仅当前设备 扫描前角膜地形图，以确定屈光引起的形状变化和通过该地形图的组织。 测试仍然需要进一步检查内皮细胞计数之前释放移植。 因此，目前的建议试图通过以下方式增加确定内皮细胞计数的能力： 修改现有设备以生产新设备，该新设备将扫描前角膜 地形图检查屈光手术的迹象，并进行内皮细胞计数。这种装置 将允许在组织采集之前筛选这两个标准， 和时间

Marous, James R. An Eye Bank Endothelial Cell Counter Abstract Procurement of donor corneal tissue for transplant use is an important social service undertaken by largely underfunded eye banks. Tissue is not only harvested, but must be screened for a myriad of issues that may make the tissue unsuitable for transplant or pose a health risk to the tissue recipient. Donor tissue may be rejected for morphological issues including prior refractive surgeries (LASIK) or low endothelial cell count. Donor tissue that has undergone a procedure leaving an epithelial flap, such as LASIK, cannot be use for transplant as the flap will separate during suturing. Low endothelial cell count is also a basis for rejection of donor tissue as low endothelial cell count is a precursor to substantial corneal edema. Currently, eye banks screen donor tissue for these issues after the tissue has been harvested either as a whole globe or a corneal-scleral rim. Tissue is returned to the eye bank laboratory and a variety of tests are performed including slit lamp examinations to determine tissue efficacy and specular microscopy to determine endothelial cell count. In a prior funded project, VOL has developed a portable eye scanner device to screen tissue for prior refractive surgeries in the field, prior to tissue harvesting. This device not only generates a definitive metric for the determination of refractive surgery presence, but also reduces the cost associated with tissue harvesting should the tissue be deemed unusable. The current device only scans anterior corneal topography for refractive induced shape changes and tissue that passes this test still requires further examination for endothelial cell count before release for transplant. The current proposal therefore seeks to add the ability to determine endothelial cell count by modifying the existing device to produce a new device that will both scan anterior corneal topography for signs of refractive surgery and perform an endothelial cell count. Such a device will allow screening of both criteria before tissue harvesting thereby saving both significant cost and time.