PL Mouse

小鼠模型和癌症干细胞计划的科学目标和中心主题是 研究干细胞功能的不同方面，包括自我更新，重编程， 去分化和分化，使用小鼠，果蝇，非洲爪蟾，和斑马鱼作为模型，与 目的是了解更多关于胚胎、组织和癌症干细胞的知识。与此相关的是， 诱导多能干细胞（iPSC）技术研究基因组重编程的机制，包括 DNA甲基化模式的变化，以了解癌症干细胞如何通过基因组 重编程，并开发人类疾病的“盘中病”模型。发育信号 正在研究经常被重新激活并用于驱动癌细胞表型的途径，包括 Wnt/β-连环蛋白途径，ERBB 2、RET和TAM受体酪氨酸激酶，以及TGF-β途径。的 开发和使用小鼠模型来研究癌症生物学和炎症在 致癌作用也是重要的目标，并且还将慢病毒载体用于癌症治疗和用于 开发新的癌症模型。 该计划包括来自八个不同实验室（部门）的十二名成员，见下文 一页的人员名单。 上一个预算年度的NCI和其他同行评审的癌症相关支持（直接成本）为 10760318美元美国国立卫生研究院和其他联邦机构对该项目的实质性支持见 外部资助的研究项目。 在上一个资助期（2008年至2010年），本计划成员发表的癌症相关出版物总数 2012年，237。在所有出版物中，7%为方案内出版物，12%为方案间出版物。

The scientific goals and central themes of the Mouse Models and Cancer Stem Cells Program are to investigate different aspects of stem cell function, including self renewal, reprogramming, and dedifferentiation and differentiation, using mouse, Drosophila, Xenopus, and zebrafish as models, with the goal of learning more about embryonic, tissue and cancer stem cells. Linked to this are major efforts to use induced pluripotent stem cell (iPSC) technology to study mechanisms of genomic reprogramming, including changes in DNA methylation patterns, to learn how cancer stem cells might arise through genomic reprogramming, and to develop "disease-in-a dish" models of human diseases. Developmental signaling pathways that are often reactivated and used to drive cancer cell phenotypes are being studied, including the Wnt/p-catenin pathway, the ERBB2, RET, and TAM receptor tyrosine kinases, and TGF-p pathways. The development and use of mouse models to study cancer biology and the role of inflammation in carcinogenesis are also important goals, and also to utilize lentivirus vectors for cancer therapy and for development of new cancer models. The program includes twelve members from eight different Laboratories (Departments), see the following page for a list of personnel. The NCI and other peer-reviewed cancer related support (direct costs) for the last budget year was $10,760,318. The substantial NIH and other federal support for this program is outlined in the table of externally funded research projects. The total number of cancer-relevant publications by members of this program in the last grant period (2008- 2012) was 237. Of the total publications, 7% were intraprogrammatic and 12% were interprogrammatic.