SR Trans

转基因和基因敲除小鼠模型已经成为索尔克研究项目的组成部分。转基因核心致力于提供使用尖端技术来创建这些模型的途径。核心服务包括将DNA构建物显微注射到单细胞期胚胎中，将基因靶向小鼠胚胎干细胞(ES)显微注射到囊胚中，慢病毒感染和注射，体外受精(IVF)，冷冻保存和小鼠品系的重新衍生。此外，Core还提供人类胚胎干细胞(HES)系的注射，并将多能干细胞系诱导到免疫缺陷小鼠中形成畸胎瘤。 转基因核心开发了新的技术和应用，使个人能够立即接触到处理转基因小鼠的知识，并提供了研究合作的潜力。自1994年以来，有100多份出版物是基于转基因核心组织创造的转基因小鼠品系。 所有CCSG支持的核心被组织到一个组：基础研究。 该共享资源在摘要ID中被归类为类别(1.03)转基因动物设施

Transgenic and knockout mouse models have become an integral part ofthe research programs at Salk. The Transgenic Core is dedicated to providing access to cutting-edge technologies to create these models. Core services include microinjection of DNA constructs into one-cell stage embryos, microinjection of gene targeted mouse embryonic stem (ES) cells into blastocysts, lentiviral infection and injection, in-vitro fertilization (IVF), cryopreservation and rederivation of mouse lines. In addition, the Core offers injection of human embryonic stem (hES) cell lines and induced pluripotent stem (IPS) cell lines into immunodeficient mice to form teratomas. The Transgenic Core develops new techniques and applications, provides immediate access to individuals with knowledge of dealing with transgenic mice, and offers the potential for research collaborations. More than 100 publications were based on genetically altered mouse lines created by the Transgenic Core since 1994. All CCSG supported core are organized into one group: Basic Research. This shared resource is categorized as category (1.03) Transgenic Animal Facility in summary ID